丹参干根多糖的结构特征及其作为疫苗佐剂诱导体液和细胞免疫反应的用途

IF 4.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Molecular Sciences Pub Date : 2024-07-16 DOI:10.3390/ijms25147765

Yixuan Zhu, Xiaochen Yang, Pengfei Gu, Xiao Wang, Yongzhan Bao, Wanyu Shi

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引用次数: 0

摘要

为了补充从丹参中提取的丹参多糖在核磁共振分析方面的研究空白，明确其作为佐剂的免疫增强作用，我们从丹参中分离纯化了由Ara、Gal、Glc等九种单糖组成的SMPD-2。其平均分子量为 37.30 ± 0.096 KDa。主链主要由→4)-α-D-Galp-(1→、→3,6)-β-D-Glcp-(1→和少量α-L-Araf-(1→组成。将 SMPD-2 作为 OVA 佐剂给小鼠皮下注射后，我们发现它能激活淋巴结中的 DCs，增加 OVA 特异性抗体分泌，刺激脾脏淋巴细胞活化，从而增强小鼠的免疫反应，并显示出良好的生物安全性。总之，SMPD-2 是一种很有前途的候选佐剂。

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The Structural Characterization of a Polysaccharide from the Dried Root of Salvia miltiorrhiza and Its Use as a Vaccine Adjuvant to Induce Humoral and Cellular Immune Responses

In order to supplement the research gap concerning Salvia miltiorrhiza polysaccharide extracted from Danshen in NMR analysis, and to clarify its immune enhancement effect as an adjuvant, we isolated and purified SMPD–2, which is composed of nine monosaccharides such as Ara, Gal, and Glc from Danshen. Its weight average molecular weight was 37.30 ± 0.096 KDa. The main chain was mainly composed of →4)-α-D-Galp-(1→, →3,6)-β-D-Glcp-(1→ and a small amount of α-L-Araf-(1→. After the subcutaneous injection of SMPD–2 as an adjuvant to OVA in mice, we found that it enhanced the immune response by activating DCs from lymph nodes, increasing OVA-specific antibody secretion, stimulating spleen lymphocyte activation, and showing good biosafety. In conclusion, SMPD–2 could be a promising candidate for an adjuvant.

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来源期刊

International Journal of Molecular Sciences Chemistry-Organic Chemistry

CiteScore

8.10

自引率

10.70%

发文量

13472

审稿时长

17.49 days

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).