造礁珊瑚热适应过程中的甲基化和转录可塑性模式

IF 3.5 2区 生物学 Q1 EVOLUTIONARY BIOLOGY
Leslie Guerrero, Rachael Bay
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引用次数: 0

摘要

表型可塑性可以使生物体在短期环境波动中得到缓冲。例如,在许多物种中,先前暴露于升高的温度可提高热耐受性。先前的研究发现,对较高温度的适应可以影响对随后急性热应力的转录反应(以下简称 "转录反应调制")。然而,介导这种基因表达反应以及最终导致表型可塑性的机制在很大程度上仍不为人所知。表观遗传修饰是调节转录反应的良好候选机制,因为它们与基因表达广泛相关。在这里,我们研究了 DNA 甲基化的变化作为控制造礁珊瑚 Acropora nana 基因表达可塑性和热适应性变化的一种可能机制。我们发现,在适应不同温度的珊瑚中,基因表达对急性应激的反应发生了改变,在预适应较高温度的珊瑚中,许多基因对热应激的反应减弱。同时,我们观察到甲基化在适应期(11 天)和急性热应激期(24 小时)的变化。我们观察到,急性热胁迫导致基因水平甲基化的变化,并引起不同基因集的急性转录反应。此外,驯化诱导的基因表达可塑性变化和不同的甲基化也主要发生在不同的基因组中。与我们最初的假设相反,差异甲基化的程度与基因表达可塑性的变化之间没有整体相关性。我们确实发现有少量基因(14 个基因)同时表现出表达反应减弱和甲基化变化,但在统计学上有显著的重叠,这可能是进一步研究的候选基因。总之,我们的研究结果表明,转录反应调节的发生与热适应所诱导的甲基化变化无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Patterns of methylation and transcriptional plasticity during thermal acclimation in a reef-building coral

Patterns of methylation and transcriptional plasticity during thermal acclimation in a reef-building coral

Phenotypic plasticity can buffer organisms against short-term environmental fluctuations. For example, previous exposure to increased temperatures can increase thermal tolerance in many species. Prior studies have found that acclimation to higher temperature can influence the magnitude of transcriptional response to subsequent acute thermal stress (hereafter, “transcriptional response modulation”). However, mechanisms mediating this gene expression response and, ultimately, phenotypic plasticity remain largely unknown. Epigenetic modifications are good candidates for modulating transcriptional response, as they broadly correlate with gene expression. Here, we investigate changes in DNA methylation as a possible mechanism controlling shifts in gene expression plasticity and thermal acclimation in the reef-building coral Acropora nana. We find that gene expression response to acute stress is altered in corals acclimated to different temperatures, with many genes exhibiting a dampened response to heat stress in corals pre-conditioned to higher temperatures. At the same time, we observe shifts in methylation during both acclimation (11 days) and acute heat stress (24 h). We observed that the acute heat stress results in shifts in gene-level methylation and elicits an acute transcriptional response in distinct gene sets. Further, acclimation-induced shifts in gene expression plasticity and differential methylation also largely occur in separate sets of genes. Counter to our initial hypothesis no overall correlation between the magnitude of differential methylation and the change in gene expression plasticity. We do find a small but statistically significant overlap in genes exhibiting both dampened expression response and shifts in methylation (14 genes), which could be candidates for further inquiry. Overall, our results suggest transcriptional response modulation occurs independently from methylation changes induced by thermal acclimation.

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来源期刊
Evolutionary Applications
Evolutionary Applications 生物-进化生物学
CiteScore
8.50
自引率
7.30%
发文量
175
审稿时长
6 months
期刊介绍: Evolutionary Applications is a fully peer reviewed open access journal. It publishes papers that utilize concepts from evolutionary biology to address biological questions of health, social and economic relevance. Papers are expected to employ evolutionary concepts or methods to make contributions to areas such as (but not limited to): medicine, agriculture, forestry, exploitation and management (fisheries and wildlife), aquaculture, conservation biology, environmental sciences (including climate change and invasion biology), microbiology, and toxicology. All taxonomic groups are covered from microbes, fungi, plants and animals. In order to better serve the community, we also now strongly encourage submissions of papers making use of modern molecular and genetic methods (population and functional genomics, transcriptomics, proteomics, epigenetics, quantitative genetics, association and linkage mapping) to address important questions in any of these disciplines and in an applied evolutionary framework. Theoretical, empirical, synthesis or perspective papers are welcome.
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