Zi-Yue Fu, Dao-Feng Zhang, Meng-Han Huang, Hong-Chuan Wang, Xiao-Ye Chen, Yu-Fang Yao, Yang Yuan, Wen-Jun Li
{"title":"从琼脂降解共培养物中分离出的两种海洋细菌:Thalassospira aquimaris sp.","authors":"Zi-Yue Fu, Dao-Feng Zhang, Meng-Han Huang, Hong-Chuan Wang, Xiao-Ye Chen, Yu-Fang Yao, Yang Yuan, Wen-Jun Li","doi":"10.1007/s10482-024-02000-9","DOIUrl":null,"url":null,"abstract":"<div><p>Two novel Gram-stain-negative, aerobic, and non-motile strains, designated FZY0004<sup>T</sup> and YYF002<sup>T</sup>, were isolated from an agar-degrading co-culture, which was obtained from seawater of the intertidal zone of Yancheng City, the Yellow Sea of China. Strain FZY0004<sup>T</sup> optimally grew at 28 °C, pH 7.0, and 2–6% NaCl, while strain YYF002<sup>T</sup> optimally grew at 28 °C, pH 7.5, and 2–4% NaCl. Strain FZY0004<sup>T</sup> possessed Q-9 as the major respiratory quinone, and its major fatty acids (> 10%) were summed feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i>), C<sub>16:0</sub>, and summed feature 3 (C<sub>16:1</sub> <i>ω</i>7<i>c</i>/C<sub>16:1</sub> <i>ω</i>6<i>c</i>). The polar lipids identified in strain FZY0004<sup>T</sup> were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and several unidentified phospholipids (PL) and lipids (L). On the other hand, strain YYF002<sup>T</sup> had MK-6 as the predominant respiratory quinone and its major fatty acids consisted of iso-C<sub>15:0</sub>, iso-C<sub>15:1</sub> G, and iso-C<sub>15:0</sub> 3-OH. The polar lipids identified in strain YYF002<sup>T</sup> were aminolipid (AL), PE, and several unidentified lipids. Strain FZY0004<sup>T</sup> shared 99.5% 16S rRNA gene sequence similarity and 90.1% average nucleotide identity (ANI) with <i>T. povalilytica</i> Zumi 95<sup>T</sup>, and strain YYF002<sup>T</sup> shared 99.2% 16S rRNA gene sequence similarity and 88.2% ANI with <i>W. poriferorum</i> JCM 12885<sup>T</sup>. The genomic DNA G + C contents of strains FZY0004<sup>T</sup> and YYF002<sup>T</sup> were 54.5% and 33.5%, respectively. The phylogenetic, phenotypic, and physiological characteristics permitted the distinction of the two strains from their neighbors, and we thus propose the names <i>Thalassospira aquimaris</i> sp. nov. (type strain FZY0004<sup>T</sup> = JCM 35895<sup>T</sup> = MCCC 1K08380<sup>T</sup>) and <i>Winogradskyella marincola</i> sp. nov. (type strain YYF002<sup>T</sup> = JCM 35950<sup>T</sup> = MCCC 1K08382<sup>T</sup>).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Thalassospira aquimaris sp. nov. and Winogradskyella marincola sp. nov. two marine bacteria isolated from an agar-degrading co-culture\",\"authors\":\"Zi-Yue Fu, Dao-Feng Zhang, Meng-Han Huang, Hong-Chuan Wang, Xiao-Ye Chen, Yu-Fang Yao, Yang Yuan, Wen-Jun Li\",\"doi\":\"10.1007/s10482-024-02000-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Two novel Gram-stain-negative, aerobic, and non-motile strains, designated FZY0004<sup>T</sup> and YYF002<sup>T</sup>, were isolated from an agar-degrading co-culture, which was obtained from seawater of the intertidal zone of Yancheng City, the Yellow Sea of China. Strain FZY0004<sup>T</sup> optimally grew at 28 °C, pH 7.0, and 2–6% NaCl, while strain YYF002<sup>T</sup> optimally grew at 28 °C, pH 7.5, and 2–4% NaCl. Strain FZY0004<sup>T</sup> possessed Q-9 as the major respiratory quinone, and its major fatty acids (> 10%) were summed feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i>), C<sub>16:0</sub>, and summed feature 3 (C<sub>16:1</sub> <i>ω</i>7<i>c</i>/C<sub>16:1</sub> <i>ω</i>6<i>c</i>). The polar lipids identified in strain FZY0004<sup>T</sup> were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and several unidentified phospholipids (PL) and lipids (L). On the other hand, strain YYF002<sup>T</sup> had MK-6 as the predominant respiratory quinone and its major fatty acids consisted of iso-C<sub>15:0</sub>, iso-C<sub>15:1</sub> G, and iso-C<sub>15:0</sub> 3-OH. The polar lipids identified in strain YYF002<sup>T</sup> were aminolipid (AL), PE, and several unidentified lipids. Strain FZY0004<sup>T</sup> shared 99.5% 16S rRNA gene sequence similarity and 90.1% average nucleotide identity (ANI) with <i>T. povalilytica</i> Zumi 95<sup>T</sup>, and strain YYF002<sup>T</sup> shared 99.2% 16S rRNA gene sequence similarity and 88.2% ANI with <i>W. poriferorum</i> JCM 12885<sup>T</sup>. The genomic DNA G + C contents of strains FZY0004<sup>T</sup> and YYF002<sup>T</sup> were 54.5% and 33.5%, respectively. The phylogenetic, phenotypic, and physiological characteristics permitted the distinction of the two strains from their neighbors, and we thus propose the names <i>Thalassospira aquimaris</i> sp. nov. (type strain FZY0004<sup>T</sup> = JCM 35895<sup>T</sup> = MCCC 1K08380<sup>T</sup>) and <i>Winogradskyella marincola</i> sp. nov. 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Thalassospira aquimaris sp. nov. and Winogradskyella marincola sp. nov. two marine bacteria isolated from an agar-degrading co-culture
Two novel Gram-stain-negative, aerobic, and non-motile strains, designated FZY0004T and YYF002T, were isolated from an agar-degrading co-culture, which was obtained from seawater of the intertidal zone of Yancheng City, the Yellow Sea of China. Strain FZY0004T optimally grew at 28 °C, pH 7.0, and 2–6% NaCl, while strain YYF002T optimally grew at 28 °C, pH 7.5, and 2–4% NaCl. Strain FZY0004T possessed Q-9 as the major respiratory quinone, and its major fatty acids (> 10%) were summed feature 8 (C18:1ω7c), C16:0, and summed feature 3 (C16:1ω7c/C16:1ω6c). The polar lipids identified in strain FZY0004T were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and several unidentified phospholipids (PL) and lipids (L). On the other hand, strain YYF002T had MK-6 as the predominant respiratory quinone and its major fatty acids consisted of iso-C15:0, iso-C15:1 G, and iso-C15:0 3-OH. The polar lipids identified in strain YYF002T were aminolipid (AL), PE, and several unidentified lipids. Strain FZY0004T shared 99.5% 16S rRNA gene sequence similarity and 90.1% average nucleotide identity (ANI) with T. povalilytica Zumi 95T, and strain YYF002T shared 99.2% 16S rRNA gene sequence similarity and 88.2% ANI with W. poriferorum JCM 12885T. The genomic DNA G + C contents of strains FZY0004T and YYF002T were 54.5% and 33.5%, respectively. The phylogenetic, phenotypic, and physiological characteristics permitted the distinction of the two strains from their neighbors, and we thus propose the names Thalassospira aquimaris sp. nov. (type strain FZY0004T = JCM 35895T = MCCC 1K08380T) and Winogradskyella marincola sp. nov. (type strain YYF002T = JCM 35950T = MCCC 1K08382T).
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.