苏云金芽孢杆菌亚种的基因组历史:从发现到今天的产品

IF 1.7 3区 农林科学 Q2 ENTOMOLOGY
Lea Schäfer, Johannes A. Jehle, Regina G. Kleespies, Jörg T. Wennmann
{"title":"苏云金芽孢杆菌亚种的基因组历史:从发现到今天的产品","authors":"Lea Schäfer, Johannes A. Jehle, Regina G. Kleespies, Jörg T. Wennmann","doi":"10.1111/jen.13325","DOIUrl":null,"url":null,"abstract":"<jats:italic>Bacillus thuringiensis</jats:italic> subsp. <jats:italic>tenebrionis</jats:italic> (Btt) was discovered in 1982 and has been used as a biological control agent against the Colorado potato beetle (<jats:italic>Leptinotarsa decemlineata</jats:italic>) since the early 1990s. In this study, we sequenced, assembled and analysed the genome of strain DSM 2803, which is the patent deposit of the original Btt isolate BI 256‐82. A high‐quality reference genome is provided that allows investigating the genomic changes throughout the commercial history of Btt. Strain DSM 2803 (BI 256‐82) is particularly interesting since the subspecies description of ‘<jats:italic>tenebrionis</jats:italic>’ is based on it and because it represents the genomic origin of the commercial Btt strain currently used in plant protection. Comparative genome analysis of six Btt strains derived from BI 256‐82 revealed that the genomes were generally highly similar. However, some plasticity in the profile of extrachromosomal elements was detected, including recombination‐dependent plasmid differences and the presence/absence of a circular plasmid‐like prophage.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The genome history of Bacillus thuringiensis subsp. tenebrionis: From discovery to present day product\",\"authors\":\"Lea Schäfer, Johannes A. Jehle, Regina G. Kleespies, Jörg T. Wennmann\",\"doi\":\"10.1111/jen.13325\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<jats:italic>Bacillus thuringiensis</jats:italic> subsp. <jats:italic>tenebrionis</jats:italic> (Btt) was discovered in 1982 and has been used as a biological control agent against the Colorado potato beetle (<jats:italic>Leptinotarsa decemlineata</jats:italic>) since the early 1990s. In this study, we sequenced, assembled and analysed the genome of strain DSM 2803, which is the patent deposit of the original Btt isolate BI 256‐82. A high‐quality reference genome is provided that allows investigating the genomic changes throughout the commercial history of Btt. Strain DSM 2803 (BI 256‐82) is particularly interesting since the subspecies description of ‘<jats:italic>tenebrionis</jats:italic>’ is based on it and because it represents the genomic origin of the commercial Btt strain currently used in plant protection. Comparative genome analysis of six Btt strains derived from BI 256‐82 revealed that the genomes were generally highly similar. However, some plasticity in the profile of extrachromosomal elements was detected, including recombination‐dependent plasmid differences and the presence/absence of a circular plasmid‐like prophage.\",\"PeriodicalId\":14987,\"journal\":{\"name\":\"Journal of Applied Entomology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-07-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Entomology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/jen.13325\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Entomology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/jen.13325","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

苏云金芽孢杆菌(Bacillus thuringiensis subsp. tenebrionis,Btt)于 1982 年被发现,自 20 世纪 90 年代初以来一直被用作防治科罗拉多马铃薯甲虫(Leptinotarsa decemlineata)的生物防治剂。在这项研究中,我们对菌株 DSM 2803 的基因组进行了测序、组装和分析,该菌株是原始 Btt 分离物 BI 256-82 的专利保存物。该研究提供了高质量的参考基因组,有助于研究 Btt 在整个商业化历史中的基因组变化。菌株 DSM 2803(BI 256-82)特别有意思,因为 "tenebrionis "亚种的描述就是基于它,而且它代表了目前用于植物保护的商业 Btt 菌株的基因组起源。对源自 BI 256-82 的六个 Btt 菌株进行的基因组比较分析表明,这些菌株的基因组总体上高度相似。然而,在染色体外元素的概况中发现了一些可塑性,包括重组依赖性质粒差异和环状质粒样原生质体的存在/不存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The genome history of Bacillus thuringiensis subsp. tenebrionis: From discovery to present day product
Bacillus thuringiensis subsp. tenebrionis (Btt) was discovered in 1982 and has been used as a biological control agent against the Colorado potato beetle (Leptinotarsa decemlineata) since the early 1990s. In this study, we sequenced, assembled and analysed the genome of strain DSM 2803, which is the patent deposit of the original Btt isolate BI 256‐82. A high‐quality reference genome is provided that allows investigating the genomic changes throughout the commercial history of Btt. Strain DSM 2803 (BI 256‐82) is particularly interesting since the subspecies description of ‘tenebrionis’ is based on it and because it represents the genomic origin of the commercial Btt strain currently used in plant protection. Comparative genome analysis of six Btt strains derived from BI 256‐82 revealed that the genomes were generally highly similar. However, some plasticity in the profile of extrachromosomal elements was detected, including recombination‐dependent plasmid differences and the presence/absence of a circular plasmid‐like prophage.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.40
自引率
5.30%
发文量
132
审稿时长
6 months
期刊介绍: The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems. Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信