吸烟引起的牛奶中β-酪蛋白的抑制与乳腺上皮细胞中 miR-210-5p 表达的增加有关

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Takeshi Chiba , Akira Takaguri , Toshiyasu Mikuma , Toshimi Kimura , Tomoji Maeda
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引用次数: 0

摘要

哺乳期吸烟会对母乳的数量和成分产生有害影响。食用含有 miR-210-5p 的母乳的婴儿患脑相关疾病的风险可能更高。我们研究了哺乳期吸烟是否会降低乳汁中β-酪蛋白的浓度,以及miR-210-5p的表达是否参与了吸烟引起的β-酪蛋白抑制。在哺乳期,母体 CD1 小鼠在烟雾室中暴露于香烟烟雾(1.7 毫克焦油和 14 毫克尼古丁),每天两次,每次 1 小时,连续五天。对照组小鼠被放置在与烟雾室大小相当的充满空气的室内,母鼠分离时间与烟熏小鼠相同。与对照组相比,哺乳期母体暴露于烟雾中会显著降低烟熏小鼠乳腺上皮中β-酪蛋白的表达。信号转导与激活转录5(STAT5)和磷酸化STAT5(pSTAT5)是参与β-酪蛋白表达的转录因子。在烟熏小鼠的乳腺上皮中,pSTAT5 和 STAT5 的水平显著低于对照组,而 miR-210-5p 的表达则显著高于对照组。miR-210-5p模拟转染的人乳腺上皮MCF-12A细胞的β-酪蛋白、pSTAT5和STAT5蛋白水平明显低于对照组siRNA转染细胞。这些结果表明,烟雾暴露通过抑制 STAT5 的表达,导致乳腺上皮细胞中 miR-210-5p 表达增加,pSTAT5 和 β-酪蛋白水平降低。此外,尼古丁以浓度依赖的方式降低了非恶性人类乳腺上皮 MCF-12A 细胞中的β-酪蛋白水平,并增加了 miR-210-5p 的表达,这表明尼古丁会显著影响母乳中的β-酪蛋白和 miR-210-5p 水平。这些结果突显了吸烟对母乳的不良影响,为医护人员和普通民众提供了重要信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Smoking-induced suppression of β-casein in milk is associated with an increase in miR-210-5p expression in mammary epithelia

Smoking during lactation harmfully affects the amount and constituents of breast milk. Infants who consume breast milk containing miR-210-5p may have a higher risk of brain-related diseases. We investigated whether smoking during lactation decreases β-casein concentrations in milk and whether miR-210-5p expression is involved in smoking-induced β-casein suppression. During lactation, maternal CD1 mice were exposed to cigarette smoke (1.7 mg of tar and 14 mg of nicotine) in a smoke chamber for 1 h twice/day for five consecutive days. Control mice were placed in an air-filled chamber equivalent in size to the smoke chamber, with maternal separation times identical to those of the smoked mice. Maternal exposure to smoke during lactation significantly decreased β-casein expression in the mammary epithelia of smoked mice compared to that of the control mice. Signal transducer and activator transcription 5 (STAT5) and phosphorylated STAT5 (pSTAT5) are transcription factors involved in β-casein expression. In the mammary epithelia of smoked mice, the pSTAT5 and STAT5 levels were significantly lower, and miR-210-5p expression was significantly higher than that of the control mice. The β-casein, pSTAT5, and STAT5 protein levels of miR-210-5p mimic-transfected human mammary epithelial MCF-12A cells were significantly lower than those of control siRNA-transfected cells. These results indicate that smoke exposure led to an increase in miR-210-5p expression in mammary epithelium and a decrease in pSTAT5 and β-casein protein levels through the inhibition of STAT5 expression. Moreover, nicotine treatment decreased β-casein protein levels and increased miR-210-5p expression in non-malignant human mammary epithelial MCF-12A cells in a concentration-dependent manner, demonstrating that nicotine significantly affects the β-casein and miR-210-5p levels of breast milk. These results highlight the adverse effects of smoking on breast milk, providing essential information for healthcare professionals and general citizens.

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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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