为研究样本安全抵达中心和设施做好准备：成功实验的秘诀。

IF 1.1 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Acta crystallographica. Section F, Structural biology communications Pub Date : 2024-08-01 Epub Date: 2024-07-11 DOI:10.1107/S2053230X24006174

Sarah E J Bowman, James Byrnes, Silvia Russi, Christina M Zimanyi

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引用次数: 0

摘要

制备用于结构生物学研究的生物大分子是一个复杂而耗时的过程。我们的目标是制备出高浓度、高纯度的产品，这些产品通常会被运往大型设施，这些设施配备有制备结晶试验样品或在同步加速器和低温电子显微镜中心进行测量的工具。本文旨在提供指导，并讨论运输生物分子样品的一般注意事项。本文还详细介绍了特定实验类型（包括基于溶液和低温的技术）的样品运输。提供这些指南的目的是希望在样品制备方面投入的时间和精力不会因为运输物流而白白浪费。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Preparing research samples for safe arrival at centers and facilities: recipes for successful experiments.

Preparation of biomacromolecules for structural biology studies is a complex and time-consuming process. The goal is to produce a highly concentrated, highly pure product that is often shipped to large facilities with tools to prepare the samples for crystallization trials or for measurements at synchrotrons and cryoEM centers. The aim of this article is to provide guidance and to discuss general considerations for shipping biomacromolecular samples. Details are also provided about shipping samples for specific experiment types, including solution- and cryogenic-based techniques. These guidelines are provided with the hope that the time and energy invested in sample preparation is not lost due to shipping logistics.

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来源期刊

Acta crystallographica. Section F, Structural biology communications BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.