用于 CRISPR/Cas 辅助生物传感的工程化 crRNA。

IF 14.3 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Trends in biotechnology Pub Date : 2024-07-08 DOI:10.1016/j.tibtech.2024.06.006

Long Ma, Minghui Lu, Jingyu Jia, Nan Wang, Yaru Li, Weipan Peng, Shuli Man

引用次数: 0

摘要

基于CRISPR/Cas的诊断（CRISPR-Dx）面临着各种挑战，包括难以检测超短核苷酸、前置扩增依赖性、交叉污染、原位检测范式不足以及不便检测非核酸靶标等。本论坛概述了解决上述问题的工程CRISPR RNA（crRNA）的进展，强调了挑战、机遇和未来方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Engineered crRNA for CRISPR/Cas-assisted biosensing.

CRISPR/Cas-based diagnostics (CRISPR-Dx) face challenges, including difficulty in detecting ultrashort nucleotides, preamplification dependency, cross-contamination, insufficiency in on-pot detection paradigms, and inconvenience in detecting non-nucleic acid targets. This forum outlines the advances in engineered CRISPR RNA (crRNA) that address the aforementioned problems, highlighting challenges, opportunities, and future directions.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Trends in biotechnology 工程技术-生物工程与应用微生物

CiteScore

28.60

自引率

1.20%

发文量

198

审稿时长

1 months

期刊介绍： Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems. The major themes that TIBTECH is interested in include: Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering) Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology) Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics) Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery) Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).