层流剖面横向扩散方法在毛细管电泳毛细管内反应中的非典型应用。

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Taťána Bržezická, Lenka Kohútová, Zdeněk Glatz
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引用次数: 0

摘要

毛细管电泳(CE)是一种功能强大的分离技术,可在生物分析化学的各个领域提供快速高效的分析。它具有许多众所周知的优点,但其中一个可能是这一应用领域最重要的优点却被忽视了。这就是可以在分离毛细管内进行 nL 级的化学和生化反应。适用于这一目的的有两种基本形式:异构和均相。在前者中,一种反应物被固定在颗粒或整体支撑物上,或直接固定在毛细管壁上,另一种反应物则被注入。在后者中,毛细管内的反应物混合基于电迁移或扩散。其中一种基于扩散的方法称为层流剖面横向扩散法,是本综述的主题。由于在 CE 中利用毛细管内反应的大多数研究都集中在酶上,而酶的研究正在不断进行和详尽的审查,因此本综述涵盖了这种方法的非典型应用,但仍然是在生物分析领域。从所展示的应用中可以看出,它们并不局限于反应,也可用于其他生化系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

Capillary electrophoresis (CE) is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterized by many well-known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility to perform chemical and biochemical reactions at the nL scale inside the separation capillary. There are two basic formats applicable for this purpose, heterogeneous and homogeneous. In the former, one reactant is immobilized onto a particle or monolithic support or directly on the capillary wall, and the other is injected. In the latter, the reactant mixing inside a capillary is based on electromigration or diffusion. One of the diffusion-based methodologies, termed Transverse Diffusion of Laminar Flow Profiles, is the subject of this review. Since most studies utilizing in-capillary reactions in CE focus on enzymes, which are being continuously and exhaustively reviewed, this review covers the atypical applications of this methodology, but still in the bioanalytical field. As can be seen from the demonstrated applications, they are not limited to reactions, but can also be utilized for other biochemical systems.

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来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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