优化公马睾丸石蜡切片的制备。

IF 2.5 4区生物学 Q3 CELL BIOLOGY

Histology and histopathology Pub Date : 2025-03-01 Epub Date: 2024-06-06 DOI:10.14670/HH-18-775

Nairag Asgenbaatar, Minna Yi, Xisheng Wang, Tseweendolmaa Ulaangerel, Yingchao Shen, Xin Wen, Ming Du, Xiaoling Dong, Yibeeltu Mengkh, Manglai Dugarjav, Gerelchimeg Bou

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引用次数: 0

摘要

石蜡切片的制备是动物组织学研究中的一项重要实验技术，决定切片质量的关键因素包括石蜡切片的脱水时间、浸蜡时间和干燥温度。成年马的睾丸组织石蜡切片在梯度脱水 6 小时、浸蜡 60 分钟并在 75 度烘箱中干燥 15 分钟后，组织结构清晰、细胞形态完整，质量更高。本研究制定的详细优化程序可简化马睾丸的组织学实验和研究。

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Optimizing the preparation of paraffin sections from stallion testes.

The preparation of paraffin sections is an important experimental technique in animal histological research, and key factors that determine the quality of a section include the dehydration time, waxing time, and drying temperature of the paraffin section. Paraffin sections obtained from testis tissue of adult horses exhibited higher quality with clear tissue structure and complete cell morphology after they underwent gradient dehydration for 6 hours, were immersed in wax for 60 minutes, and were dried in a 75-degree oven for 15 minutes. The detailed, optimized procedures that are developed in the current study may simplify histological experiments and research on equine testes.

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来源期刊

Histology and histopathology 生物-病理学

CiteScore

3.90

自引率

0.00%

发文量

232

审稿时长

2 months

期刊介绍： HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.