HPE1 RNA 结合蛋白调节叶绿体 RNA 编辑,促进拟南芥在冷胁迫下的光合作用。

IF 3.5 4区 生物学 Q1 Biochemistry, Genetics and Molecular Biology
Yajun Zeng, Jie Dong, Danni Fu, Meihui Shi, Zhifeng Zheng, Mingxi Zhong, Hong-Bin Wang, Su-Juan Duan, Hong-Lei Jin
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引用次数: 0

摘要

冷胁迫对植物生长和作物产量有严重的负面影响。在这里,我们报告了一种拟南芥突变体,该突变体缺乏编码 RNA 结合蛋白的 HPE1 基因,但在冷胁迫下仍能保持较高的光合作用活性,以及较高的类叶绿体蛋白积累。我们发现 HPE1 与 MORF2 和 MORF9 相互作用,从而介导叶绿体中的 RNA 编辑。在冷胁迫下,HPE1功能的缺失提高了rpoC-488、ndhB-149、ndhB-746和matK-706四个RNA编辑位点的编辑效率,并改变了核光合作用相关基因和冷响应基因的表达。我们认为 HPE1 介导的 RNA 编辑是逆行信号转导的触发器,影响了冷胁迫下的光合作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The HPE1 RNA-binding protein modulates chloroplast RNA editing to promote photosynthesis under cold stress in Arabidopsis

The HPE1 RNA-binding protein modulates chloroplast RNA editing to promote photosynthesis under cold stress in Arabidopsis

Cold stress has severe negative consequences for plant growth and crop yield. Here, we report that an Arabidopsis thaliana mutant that lacks the HPE1 gene, which encodes an RNA-binding protein, maintains higher photosynthetic activity under cold stress, together with higher accumulation of thylakoid proteins. We showed that HPE1 interacts with MORF2 and MORF9 and thereby mediates RNA editing in chloroplasts. Loss of HPE1 function increased the editing efficiency at four RNA editing sites, rpoC-488, ndhB-149, ndhB-746 and matK-706, under cold stress and altered the expression of nuclear photosynthesis-related genes and cold-responsive genes. We propose that HPE1-mediated RNA editing acts as a trigger for retrograde signaling that affects photosynthesis under cold stress.

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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
7.00
自引率
2.90%
发文量
303
审稿时长
1.0 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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