Gabriella Rodrigues Gonçalves, Marciele Souza da Silva, Layrana Azevedo dos Santos, Thomas Zacarone Afonso Guimarães, Gabriel Bonan Taveira, Felipe Astolpho Almeida, Sarah Rodrigues Ferreira, Antonia Elenir Amancio Oliveira, Celso Shiniti Nagano, Renata Pinheiro Chaves, Vanildo Silveira, André de Oliveira Carvalho, Rosana Rodrigues and Valdirene Moreira Gomes*,
{"title":"具有几丁质结合特性的新型脂质转移蛋白的结构和功能特征:从蛋白质结构预测、分子对接和抗真菌活性中获得的启示","authors":"Gabriella Rodrigues Gonçalves, Marciele Souza da Silva, Layrana Azevedo dos Santos, Thomas Zacarone Afonso Guimarães, Gabriel Bonan Taveira, Felipe Astolpho Almeida, Sarah Rodrigues Ferreira, Antonia Elenir Amancio Oliveira, Celso Shiniti Nagano, Renata Pinheiro Chaves, Vanildo Silveira, André de Oliveira Carvalho, Rosana Rodrigues and Valdirene Moreira Gomes*, ","doi":"10.1021/acs.biochem.4c00124","DOIUrl":null,"url":null,"abstract":"<p >Faced with the emergence of multiresistant microorganisms that affect human health, microbial agents have become a serious global threat, affecting human health and plant crops. Antimicrobial peptides have attracted significant attention in research for the development of new microbial control agents. This work’s goal was the structural characterization and analysis of antifungal activity of chitin-binding peptides from <i>Capsicum baccatum</i> and <i>Capsicum frutescens</i> seeds on the growth of <i>Candida</i> and <i>Fusarium</i> species. Proteins were initially submitted to extraction in phosphate buffer pH 5.4 and subjected to chitin column chromatography. Posteriorly, two fractions were obtained for each species, <i>Cb</i>-F1 and <i>Cf</i>-F1 and <i>Cb</i>-F2 and <i>Cf</i>-F2, respectively. The <i>Cb</i>-F1 (<i>C. baccatum</i>) and <i>Cf</i>-F1 (<i>C. frutescens</i>) fractions did not bind to the chitin column. The electrophoresis results obtained after chromatography showed two major protein bands between 3.4 and 14.2 kDa for <i>Cb</i>-F2. For <i>Cf</i>-F2, three major bands were identified between 6.5 and 14.2 kDa. One band from each species was subjected to mass spectrometry, and both bands showed similarity to nonspecific lipid transfer protein. <i>Candida albicans</i> and <i>Candida tropicalis</i> had their growth inhibited by <i>Cb</i>-F2. <i>Cf</i>-F2 inhibited the development of <i>C. albicans</i> but did not inhibit the growth of <i>C. tropicalis</i>. Both fractions were unable to inhibit the growth of <i>Fusarium</i> species. The toxicity of the fractions was tested in vivo on <i>Galleria mellonella</i> larvae, and both showed a low toxicity rate at high concentrations. As a result, the fractions have enormous promise for the creation of novel antifungal compounds.</p>","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acs.biochem.4c00124","citationCount":"0","resultStr":"{\"title\":\"Structural and Functional Characterization of New Lipid Transfer Proteins with Chitin-Binding Properties: Insights from Protein Structure Prediction, Molecular Docking, and Antifungal Activity\",\"authors\":\"Gabriella Rodrigues Gonçalves, Marciele Souza da Silva, Layrana Azevedo dos Santos, Thomas Zacarone Afonso Guimarães, Gabriel Bonan Taveira, Felipe Astolpho Almeida, Sarah Rodrigues Ferreira, Antonia Elenir Amancio Oliveira, Celso Shiniti Nagano, Renata Pinheiro Chaves, Vanildo Silveira, André de Oliveira Carvalho, Rosana Rodrigues and Valdirene Moreira Gomes*, \",\"doi\":\"10.1021/acs.biochem.4c00124\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Faced with the emergence of multiresistant microorganisms that affect human health, microbial agents have become a serious global threat, affecting human health and plant crops. Antimicrobial peptides have attracted significant attention in research for the development of new microbial control agents. This work’s goal was the structural characterization and analysis of antifungal activity of chitin-binding peptides from <i>Capsicum baccatum</i> and <i>Capsicum frutescens</i> seeds on the growth of <i>Candida</i> and <i>Fusarium</i> species. Proteins were initially submitted to extraction in phosphate buffer pH 5.4 and subjected to chitin column chromatography. Posteriorly, two fractions were obtained for each species, <i>Cb</i>-F1 and <i>Cf</i>-F1 and <i>Cb</i>-F2 and <i>Cf</i>-F2, respectively. The <i>Cb</i>-F1 (<i>C. baccatum</i>) and <i>Cf</i>-F1 (<i>C. frutescens</i>) fractions did not bind to the chitin column. The electrophoresis results obtained after chromatography showed two major protein bands between 3.4 and 14.2 kDa for <i>Cb</i>-F2. For <i>Cf</i>-F2, three major bands were identified between 6.5 and 14.2 kDa. One band from each species was subjected to mass spectrometry, and both bands showed similarity to nonspecific lipid transfer protein. <i>Candida albicans</i> and <i>Candida tropicalis</i> had their growth inhibited by <i>Cb</i>-F2. <i>Cf</i>-F2 inhibited the development of <i>C. albicans</i> but did not inhibit the growth of <i>C. tropicalis</i>. Both fractions were unable to inhibit the growth of <i>Fusarium</i> species. The toxicity of the fractions was tested in vivo on <i>Galleria mellonella</i> larvae, and both showed a low toxicity rate at high concentrations. As a result, the fractions have enormous promise for the creation of novel antifungal compounds.</p>\",\"PeriodicalId\":28,\"journal\":{\"name\":\"Biochemistry Biochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-07-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.acs.org/doi/epdf/10.1021/acs.biochem.4c00124\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry Biochemistry\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.biochem.4c00124\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry Biochemistry","FirstCategoryId":"1","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.biochem.4c00124","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Structural and Functional Characterization of New Lipid Transfer Proteins with Chitin-Binding Properties: Insights from Protein Structure Prediction, Molecular Docking, and Antifungal Activity
Faced with the emergence of multiresistant microorganisms that affect human health, microbial agents have become a serious global threat, affecting human health and plant crops. Antimicrobial peptides have attracted significant attention in research for the development of new microbial control agents. This work’s goal was the structural characterization and analysis of antifungal activity of chitin-binding peptides from Capsicum baccatum and Capsicum frutescens seeds on the growth of Candida and Fusarium species. Proteins were initially submitted to extraction in phosphate buffer pH 5.4 and subjected to chitin column chromatography. Posteriorly, two fractions were obtained for each species, Cb-F1 and Cf-F1 and Cb-F2 and Cf-F2, respectively. The Cb-F1 (C. baccatum) and Cf-F1 (C. frutescens) fractions did not bind to the chitin column. The electrophoresis results obtained after chromatography showed two major protein bands between 3.4 and 14.2 kDa for Cb-F2. For Cf-F2, three major bands were identified between 6.5 and 14.2 kDa. One band from each species was subjected to mass spectrometry, and both bands showed similarity to nonspecific lipid transfer protein. Candida albicans and Candida tropicalis had their growth inhibited by Cb-F2. Cf-F2 inhibited the development of C. albicans but did not inhibit the growth of C. tropicalis. Both fractions were unable to inhibit the growth of Fusarium species. The toxicity of the fractions was tested in vivo on Galleria mellonella larvae, and both showed a low toxicity rate at high concentrations. As a result, the fractions have enormous promise for the creation of novel antifungal compounds.
期刊介绍:
Biochemistry provides an international forum for publishing exceptional, rigorous, high-impact research across all of biological chemistry. This broad scope includes studies on the chemical, physical, mechanistic, and/or structural basis of biological or cell function, and encompasses the fields of chemical biology, synthetic biology, disease biology, cell biology, nucleic acid biology, neuroscience, structural biology, and biophysics. In addition to traditional Research Articles, Biochemistry also publishes Communications, Viewpoints, and Perspectives, as well as From the Bench articles that report new methods of particular interest to the biological chemistry community.