日本白玉菇(Tremella yokohamensis)的生命周期和交配相容性

IF 1.5 4区 生物学 Q4 MYCOLOGY
Nanthawan Kaeoniwong, Kozue Sotome, Tsuyoshi Ichiyanagi, Norihiro Shimomura, Tadanori Aimi
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引用次数: 0

摘要

本研究通过对 rDNA-ITS 区域进行系统发育分析,确定在日本鸟取县采集的白玉菇为横滨担子菌(Tremella yokohamensis)。利用 4',6-二脒基-2-苯基吲哚染色法对每个细胞的细胞核进行荧光显微分析后发现,从子实体中分离出的基原孢子是单核的。此外,单核酵母菌从这些基生孢子中发芽,产生的杂交菌丝是二核的,并带有钳形细胞,这表明该物种的生命周期是异型的。从同一子实体中分离出的相容酵母菌株(如 TUFC 101924 和 TUFC 101925)在卡哥美蔬菜汁琼脂培养基上培养 7 d 后,成功地诱导了带有钳状连接的丝状阶段的发育。利用 15 个基生孢子分离物进行的交配相容性测试表明,该真菌具有双极交配系统。结果表明,横滨蘑菇是一种异花双孢蘑菇。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Life cycle and mating compatibility in the Japanese white jelly mushroom, Tremella yokohamensis

In this study, white jelly mushrooms that were collected in Tottori Prefecture, Japan, were identified as Tremella yokohamensis by phylogenetic analysis of the rDNA-ITS region. Fluorescent microscopic analysis using 4’,6-diamidino-2-phenylindole staining to visualize the nuclei in each cell revealed that basidiospores isolated from the fruiting body were monokaryotic. Furthermore, monokaryotic yeasts were germinated from these basidiospores and the resulting crossed mycelium was dikaryotic and bore clamp cells, suggesting a heterothallic lifecycle for this species. Crossing between compatible yeast strains, such as TUFC 101924 and TUFC 101925, that were isolated from the same fruiting body, successfully induced development of the filamentous stage bearing clamp connections after 7 d of incubation on Kagome vegetable juice agar medium. Mating compatibility tests employing 15 basidiospore isolates revealed that this fungus possess a bipolar mating system. The results indicated that T. yokohamensis is a heterothallic and bipolar mushroom.

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来源期刊
Mycoscience
Mycoscience MYCOLOGY-
CiteScore
3.00
自引率
7.10%
发文量
32
审稿时长
3 months
期刊介绍: Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.
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