Leonardo Bandeira, Christiana Faria, Fernando Cavalcante, Ariel Mesquita, Claudia Martins, Suzana Martins
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In total, 543 operational taxonomic units (OTUs) across 14 phyla were detected, with Actinobacteria (41.2%), Proteobacteria (26.5%), and Acidobacteria (14.3%) being the most abundant. Within Actinobacteria, 107 OTUs were found, primarily from the families Mycobacteriaceae, Pseudonocardiaceae, and Streptomycetaceae. Mycobacterium and Streptomyces were the predominant genera across all samples. Seventeen rare OTUs with relative abundance < 0.1% were identified, with 82.3% found in only one sample yet 25.5% detected in all units. Notable rare and transient genera included Salinibacterium, Nocardia, Actinomycetospora_01, Saccharopolyspora, Sporichthya, and Nonomuraea. The high diversity and distribution of Actinobacteria OTUs indicate the area's potential for discovering new rare species. Intensified prospection on underexplored environments and characterization of their actinobacterial diversity could lead to the discovery of new species capable of generating innovative natural products.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metabarcoding expands knowledge on diversity and ecology of rare actinobacteria in the Brazilian Cerrado.\",\"authors\":\"Leonardo Bandeira, Christiana Faria, Fernando Cavalcante, Ariel Mesquita, Claudia Martins, Suzana Martins\",\"doi\":\"10.1007/s12223-024-01184-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Rare and unknown actinobacteria from unexplored environments have the potential to produce new bioactive molecules. 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引用次数: 0
摘要
未开发环境中的稀有和未知放线菌有可能产生新的生物活性分子。本研究旨在利用 16 s rRNA 代谢编码确定放线菌群落的组成,尤其关注巴西塞拉多地区一个名为 Sete Cidades 国家公园的自然保护区内的稀有和未描述物种。由于这是一个未经适当法律授权而无法进入的地区,对其研究不足,因此尚未充分了解其多样性和生物技术潜力,而且该地区可能蕴藏着具有突破性遗传潜力的物种。共检测到 14 个门类的 543 个操作分类单元(OTU),其中放线菌(41.2%)、变形菌(26.5%)和酸细菌(14.3%)数量最多。在放线菌中发现了 107 个 OTU,主要来自分枝杆菌科、假心胞菌科和链霉菌科。分枝杆菌属和链霉菌属是所有样本中的主要属。17个稀有OTU相对丰度为
Metabarcoding expands knowledge on diversity and ecology of rare actinobacteria in the Brazilian Cerrado.
Rare and unknown actinobacteria from unexplored environments have the potential to produce new bioactive molecules. This study aimed to use 16 s rRNA metabarcoding to determine the composition of the actinobacterial community, particularly focusing on rare and undescribed species, in a nature reserve within the Brazilian Cerrado called Sete Cidades National Park. Since this is an inaccessible area without due legal authorization, it is understudied, and, therefore, its diversity and biotechnological potential are not yet fully understood, and it may harbor species with groundbreaking genetic potential. In total, 543 operational taxonomic units (OTUs) across 14 phyla were detected, with Actinobacteria (41.2%), Proteobacteria (26.5%), and Acidobacteria (14.3%) being the most abundant. Within Actinobacteria, 107 OTUs were found, primarily from the families Mycobacteriaceae, Pseudonocardiaceae, and Streptomycetaceae. Mycobacterium and Streptomyces were the predominant genera across all samples. Seventeen rare OTUs with relative abundance < 0.1% were identified, with 82.3% found in only one sample yet 25.5% detected in all units. Notable rare and transient genera included Salinibacterium, Nocardia, Actinomycetospora_01, Saccharopolyspora, Sporichthya, and Nonomuraea. The high diversity and distribution of Actinobacteria OTUs indicate the area's potential for discovering new rare species. Intensified prospection on underexplored environments and characterization of their actinobacterial diversity could lead to the discovery of new species capable of generating innovative natural products.
期刊介绍:
Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.