Actinoplanes kirromycinicus sp.

IF 2.1 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Antibiotics Pub Date : 2024-06-26 DOI:10.1038/s41429-024-00756-w

Jiahao Zeng, Yohei Iizaka, Moriyuki Hamada, Aya Iwai, Riku Takeuchi, Atsushi Fukumoto, Tomohiko Tamura, Yojiro Anzai

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引用次数: 0

摘要

从日本东京新宿采集的土壤样本中分离出一种新型放线菌，命名为 TPMA0078T。16S rRNA 基因序列分析表明，菌株 TPMA0078T 属于放线菌属，与 Actinoplanes regularis IFO 12514T 关系密切（16S rRNA 基因序列相似度为 99.86%）。TPMA0078T 菌株的孢子具有运动性，孢子囊呈圆柱形。TPMA0078T 菌株细胞壁肽聚糖中的二氨基酸为中二氨基亚硒酸和 3OH-meso-diaminopimelic acid。全细胞糖类为葡萄糖和甘露糖，半乳糖为次要成分。已确定的主要细胞脂肪酸是异-C15:0、异-C16:0 和前异-C17:0。主要的脑醌是 MK-9（H4），主要的极性脂质是磷脂酰乙醇胺。菌株 TPMA0078T 的这些趋化特性与放线菌一致。同时，数字 DNA-DNA 杂交和平均核苷酸同一性值显示，菌株 TPMA0078T 与 A. regularis NBRC 12514T 的亲缘关系较低。此外，菌株 TPMA0078T 的几种表型特性也与近缘种有所区别。根据其基因型和表型特征，菌株 TPMA0078T 代表了放线菌属的一个新物种，并将其命名为 Actinoplanes kirromycinicus sp.模式菌株为 TPMA0078T（=NBRC 116422T = TBRC 18262T）。

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Actinoplanes kirromycinicus sp. nov., isolated from soil

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Actinoplanes kirromycinicus sp. nov., isolated from soil

A novel actinomycete, designated as TPMA0078T, was isolated from a soil sample collected in Shinjuku, Tokyo, Japan. 16S rRNA gene sequence analysis indicated that strain TPMA0078T belongs to the genus Actinoplanes and is closely related to Actinoplanes regularis IFO 12514T (99.86% 16S rRNA gene sequence similarity). The spores of strain TPMA0078T were motile, and the sporangia were cylindrical. The diamino acids in the cell wall peptidoglycan of strain TPMA0078T were meso-diaminopimelic acid and 3OH-meso-diaminopimelic acid. Whole-cell sugars were glucose and mannose, with galactose as a minor component. The major cellular fatty acids identified were iso-C15:0, iso-C16:0, and anteiso-C17:0. The predominant menaquinone was MK-9(H4), and the principal polar lipid was phosphatidylethanolamine. These chemotaxonomic properties of strain TPMA0078T were consistent with those of Actinoplanes. Meanwhile, digital DNA–DNA hybridization and average nucleotide identity values showed low relatedness between strain TPMA0078T and A. regularis NBRC 12514T. Furthermore, several phenotypic properties of strain TPMA0078T distinguished it from those of closely related species. Based on its genotypic and phenotypic characteristics, strain TPMA0078T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes kirromycinicus sp. nov. is proposed. The type strain is TPMA0078T (=NBRC 116422T = TBRC 18262T).

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来源期刊

Journal of Antibiotics 医学-免疫学

CiteScore

6.60

自引率

3.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Antibiotics seeks to promote research on antibiotics and related types of biologically active substances and publishes Articles, Review Articles, Brief Communication, Correspondence and other specially commissioned reports. The Journal of Antibiotics accepts papers on biochemical, chemical, microbiological and pharmacological studies. However, studies regarding human therapy do not fall under the journal’s scope. Contributions regarding recently discovered antibiotics and biologically active microbial products are particularly encouraged. Topics of particular interest within the journal''s scope include, but are not limited to, those listed below: Discovery of new antibiotics and related types of biologically active substances Production, isolation, characterization, structural elucidation, chemical synthesis and derivatization, biological activities, mechanisms of action, and structure-activity relationships of antibiotics and related types of biologically active substances Biosynthesis, bioconversion, taxonomy and genetic studies on producing microorganisms, as well as improvement of production of antibiotics and related types of biologically active substances Novel physical, chemical, biochemical, microbiological or pharmacological methods for detection, assay, determination, structural elucidation and evaluation of antibiotics and related types of biologically active substances Newly found properties, mechanisms of action and resistance-development of antibiotics and related types of biologically active substances.