中心体组织的质膜内折与皮质肌动蛋白结构域的生长有关。

IF 7.4 1区 生物学 Q1 CELL BIOLOGY
Journal of Cell Biology Pub Date : 2024-10-07 Epub Date: 2024-06-27 DOI:10.1083/jcb.202403115
Rebecca Tam, Tony J C Harris
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引用次数: 0

摘要

调节细胞形状的变化需要诱导皮质细胞骨架结构域。这通常涉及质膜(PM)形貌的局部变化。中心体组织皮质结构域,可通过局部向内拉动质膜来影响质膜的形貌。这些中心体效应是耦合的吗?在合胞果蝇胚胎皮层,中心体诱导的肌动蛋白帽生长为有丝分裂的圆顶状区室。我们发现新生帽是星形中心体 MT 阵列上形成的 PM 褶皱和小管的集合。局部的折叠需要中心体和动力蛋白的活动,而肌球蛋白的表面张力会阻止它们在其他地方形成。中心体参与的PM内陷通过Arp2/3诱导途径变得特别丰富。褶皱之间的 Arp2/3 肌动蛋白网络生长抵消了中心体的拉力,并分散了褶皱以促进肌动蛋白帽的扩张。中心粒或 Arp2/3 中断导致的异常结构域拓扑与外ocytic囊泡结合减少有关。总之,我们的数据表明,中心体组织的 PM 折叠协调了 Arp2/3 网络的生长和皮质结构域组装的外泌作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Centrosome-organized plasma membrane infoldings linked to growth of a cortical actin domain.

Regulated cell shape change requires the induction of cortical cytoskeletal domains. Often, local changes to plasma membrane (PM) topography are involved. Centrosomes organize cortical domains and can affect PM topography by locally pulling the PM inward. Are these centrosome effects coupled? At the syncytial Drosophila embryo cortex, centrosome-induced actin caps grow into dome-like compartments for mitoses. We found the nascent cap to be a collection of PM folds and tubules formed over the astral centrosomal MT array. The localized infoldings require centrosome and dynein activities, and myosin-based surface tension prevents them elsewhere. Centrosome-engaged PM infoldings become specifically enriched with an Arp2/3 induction pathway. Arp2/3 actin network growth between the infoldings counterbalances centrosomal pulling forces and disperses the folds for actin cap expansion. Abnormal domain topography with either centrosome or Arp2/3 disruption correlates with decreased exocytic vesicle association. Together, our data implicate centrosome-organized PM infoldings in coordinating Arp2/3 network growth and exocytosis for cortical domain assembly.

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来源期刊
Journal of Cell Biology
Journal of Cell Biology 生物-细胞生物学
CiteScore
12.60
自引率
2.60%
发文量
213
审稿时长
1 months
期刊介绍: The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.
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