曼氏螺旋绦虫(绦虫纲:Diphyllobothriidae)的 Annexin 基因家族及其在具有医学意义的扁形动物中的系统发育模式。

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2024-01-01 Epub Date: 2024-06-21 DOI:10.1051/parasite/2024034
Xiao Yi Su, Fei Gao, Si Yao Wang, Jing Li, Zhong Quan Wang, Xi Zhang
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引用次数: 0

摘要

曼氏螺旋体幼虫是人类和动物麻风病的病原体。附件蛋白是在寄生虫体内发挥重要作用的蛋白质。然而,我们对曼氏螺旋体中的附件蛋白的了解仍然不足。本研究鉴定了曼氏沙门氏菌中 18 个附件蛋白(ANX)家族的新成员。聚类分析表明,所有的 SmANXs 都被分为两大类,这与保守的图案组织模式是一致的。18 个 SmANXs 在所有发育阶段(稚虫期、成虫期和卵期)都能被检测到,并且在所研究的所有组织/器官中显示出无处不在但高度可变的表达模式。代表性成员 rSmANX18 已被成功克隆和表达。该蛋白被免疫定位在纤毛虫的皮层和实质组织中,以及成虫的皮层、实质组织、子宫和卵壳中。重组蛋白能以 Ca2+ 依赖性方式与磷脂高亲和力结合,显示出很高的抗凝血活性,并能与 FITC 结合识别凋亡细胞。在绦虫和吸虫中都发现了Annexin基因的多态性和保守的核心基序变异。SmANXs 还揭示了具有医学价值的扁形动物的高度遗传多样性。我们的发现为进一步研究曼氏沙门氏菌以及其他存在 ANXs 的类群中 ANXs 的生物功能奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Annexin gene family in Spirometra mansoni (Cestoda: Diphyllobothriidae) and its phylogenetic pattern among Platyhelminthes of medical interest.

The plerocercoid larvae of Spirometra mansoni are etiological agents of human and animal sparganosis. Annexins are proteins with important roles in parasites. However, our knowledge of annexins in S. mansoni is still inadequate. In this study, 18 new members of the Annexin (ANX) family were characterized in S. mansoni. The clustering analysis demonstrated that all the SmANXs were divided into two main classes, consistent with the patterns of conserved motif organization. The 18 SmANXs were detected at all developmental stages (plerocercoid, adult, and egg) and displayed ubiquitous but highly variable expression patterns in all tissues/organs studied. The representative member rSmANX18 was successfully cloned and expressed. The protein was immunolocalized in the tegument and parenchyma of the plerocercoid and in the tegument, parenchyma, uterus and egg shell of adult worms. The recombinant protein can bind phospholipids with high affinity in a Ca2+-dependent manner, shows high anticoagulant activity and combines with FITC to recognize apoptotic cells. Annexin gene polymorphism and conservative core motif permutation were found in both cestodes and trematodes. SmANXs also revealed high genetic diversity among Platyhelminthes of medical interest. Our findings lay a foundation for further studies on the biological functions of ANXs in S. mansoni as well as other taxa in which ANXs occur.

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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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