桃金娘叶甲醇提取物软膏对耐甲氧西林金黄色葡萄球菌诱发的大鼠烧伤伤口感染的抗菌、消炎和伤口愈合作用

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioMed Research International Pub Date : 2024-06-11 eCollection Date: 2024-01-01 DOI:10.1155/2024/6758817
Zohre Jafari, Hassan Bardania, Mehrzad Jafari Barmak, Saba Eslami, Yaser Mahmoudi-Mourderaz, Narges Roustaei, Mohammad Sharif Talebianpoor, Esmaeel Panahi Kokhdan, Seyed Sajjad Khoramrooz
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引用次数: 0

摘要

材料和方法在一项研究实验中,48 只雄性 Wistar 大鼠被麻醉,背部二度烧伤。然后在大鼠伤口处均匀接种 MRSA。每天在烧伤伤口上涂抹不同的治疗剂,包括桃金娘软膏、纳米银颗粒、纳米银颗粒-桃金娘软膏、磺胺嘧啶银-桃金娘软膏、1% 磺胺嘧啶银、莫匹罗星软膏和阳性对照。研究测量了大鼠血清中的抗菌效果、伤口面积、伤口愈合百分比、抗氧化能力、丙二醛和一氧化氮浓度。使用 GraphPad 软件进行数据分析,用单向方差分析和 Tukey 检验来确定结果的统计学意义:结果:与阳性对照组、纳米银颗粒软膏和磺胺嘧啶银相比,使用桃金娘软膏、纳米银颗粒-桃金娘软膏和莫匹罗星治疗的大鼠减少了细菌生长(P < 0.05)。与其他组相比,桃金娘软膏组的伤口面积在第七天和第十四天显著减少,MDA 和一氧化氮的水平也显著降低。与其他组相比,桃金娘软膏和磺胺嘧啶银组的表皮和真皮厚度有所增加:基于桃金娘的抗炎、抗菌和伤口愈合特性,经过进一步研究,这种植物的软膏可用作 MRSA 引起的烧伤伤口感染的主要或辅助治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial, Anti-inflammatory, and Wound Healing Properties of Myrtus communis Leaf Methanolic Extract Ointment on Burn Wound Infection Induced by Methicillin-Resistant Staphylococcus aureus in Rats.

Materials and methods: In a research experiment, 48 male Wistar rats were anesthetized and second-degree burns were induced on their backs. The rats' wounds were then uniformly inoculated with MRSA. Various treatments were applied to the burn wounds daily, including Myrtus ointment, silver nanoparticles, silver nanoparticles-Myrtus ointment, silver sulfadiazine-Myrtus ointment, silver sulfadiazine 1%, mupirocin ointment, and a positive control. The study measured the antimicrobial effects, wound area, percentage of wound healing, antioxidant capacities, malondialdehyde, and nitric oxide concentrations in the serum of the rats. Data analysis was performed using GraphPad software, with one-way ANOVA and Tukey's tests used to determine the statistical significance of the results.

Results: Rats treated with Myrtus ointment, silver nanoparticles-Myrtus ointment, and mupirocin had reduced bacterial growth compared to the positive control group, nanoparticle ointment, and silver sulfadiazine (P < 0.05). The wound area of the Myrtus ointment group decreased significantly on the seventh and fourteenth days, as well as the level of MDA and nitric oxide, compared to the other groups. In Myrtus and silver sulfadiazine-Myrtus ointment increased the thickness of the epidermis and dermis compared to the other groups.

Conclusion: Based on the anti-inflammatory, antimicrobial, and wound healing properties of Myrtus, with further studies, an ointment of this plant may be used as a main or complementary treatment for burn wound infections caused by MRSA.

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来源期刊
BioMed Research International
BioMed Research International BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
6.70
自引率
0.00%
发文量
1942
审稿时长
19 weeks
期刊介绍: BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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