设计和合成基于肽的法定人数感应调制器。

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-04-27 DOI:10.1016/bs.mie.2024.04.017
Xiaotian Gong, Carter J Brand, Michael A Bertucci
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引用次数: 0

摘要

法定量感应(QS)是一种依赖密度的细菌通讯系统,它使用小分子作为调控调节剂。对这些分子进行合成改变可以上调或下调该系统,从而控制表型,如能力和毒力因子的产生,这对人类健康有影响。本章阐述了一种设计和筛选合成自体诱导肽(AIP)以发现 QS SAR 的方法。此外,还详细介绍了线性和环状 AIPs 的合成、纯化和分析程序。这包括潜在合成难题的解决方案,包括使用 N-甲基氨基酸时二酮哌嗪的形成,以及含有 N 端半胱氨酸残基的肽的环化。这些程序已经并正在用于开发肺炎链球菌、蜡样芽孢杆菌、戈登链球菌和植物乳杆菌的强效 QS 调节剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Designing and synthesizing peptide-based quorum sensing modulators.

Quorum sensing (QS) is a density-dependent bacterial communication system that uses small molecules as regulatory modulators. Synthetic changes to these molecules can up-or-down-regulate this system, leading to control of phenotypes, like competence and virulence factor production, that have implications in human health. In this chapter, a methodology for library design and screening of synthetic autoinducing peptides (AIPs) to uncover QS SARs is delineated. Additionally, procedures for the synthesis, purification and analysis of linear and cyclic AIPs are detailed. This includes solutions for potential synthetic challenges including diketopiperazine formation when using N-methyl amino acids and cyclization of peptides containing N-terminal cysteine residues. These procedures have and are currently being applied to develop potent QS modulators in Streptococcus pneumoniae, Bacillus cereus, Streptococcus gordonii and Lactiplantibacillus plantarum.

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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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