利用核磁共振对细胞内致病细菌进行代谢分析

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3890-3_6

Ke-Chuan Wang, Pernille Rose Jensen

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引用次数: 0

摘要

病原体的增殖和毒性取决于可用的营养物质，当病原体从宿主细胞外（细胞外）转移到宿主细胞内（细胞内）时，营养物质也会发生变化。核磁共振（NMR）是一种用途广泛的分析方法，可用于新陈代谢研究。在本章中，我们将介绍如何将 1H NMR 与细胞感染模型相结合，研究细菌病原体及其宿主在细胞外和细胞内的代谢串扰。使用稳定同位素 13C 标记的葡萄糖突出了中心碳代谢。

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Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR.

Pathogen proliferation and virulence depend on available nutrients, and these vary when the pathogen moves from outside of the host cell (extracellular) to the inside of the host cell (intracellular). Nuclear Magnetic Resonance (NMR) is a versatile analytical method, which lends itself for metabolic studies. In this chapter, we describe how ¹H NMR can be combined with a cellular infection model to study the metabolic crosstalk between a bacterial pathogen and its host both in the extracellular and intracellular compartments. Central carbon metabolism is highlighted by using glucose labeled with the stable isotope ¹³C.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.