Hanieh Hadady, Arefin Alam, Indu Khurana, Isha Mutreja, Dhiraj Kumar, Mamilla Ravi Shankar, Rupak Dua
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引用次数: 0
摘要
由于局部细菌感染，骨科和牙科植入物的失败仍然是一个令人严重关切的问题。以往的研究试图通过改变种植体表面的质地和粗糙度或在表面涂抹抗生素来改善种植体表面，从而增强抗菌性能，延长种植体的使用寿命。然而，这些方法的效果有限。在本研究中，我们受蝉翼纳米结构的启发，尝试使用碱性水热处理（AHT）技术在纳米尺度上对钛（Ti）合金表面进行生物模拟设计，以同时赋予其抗菌特性并支持哺乳动物细胞的粘附和增殖。在 230 °C 的 1 N NaOH 溶液中分别进行了 4 小时和 8 小时的碱性水热处理，然后在 600 °C 下进行了 2 小时的后煅烧，从而形成了两种改性钛表面。我们发现，对照组的钛板表面相对光滑，而处理组（4 小时和 8 小时 AHT）则显示出含有随机分布的纳米尖峰的纳米花结构。结果表明，处理组的接触角在统计学上显著减小，从而提高了润湿特性。8 小时 AHT 组的润湿性最高，粗糙度 0.72 ± 0.08 µm 显著增加（P＜0.05）。
Optimizing alkaline hydrothermal treatment for biomimetic smart metallic orthopedic and dental implants
Orthopedic and dental implant failure continues to be a significant concern due to localized bacterial infections. Previous studies have attempted to improve implant surfaces by modifying their texture and roughness or coating them with antibiotics to enhance antibacterial properties for implant longevity. However, these approaches have demonstrated limited effectiveness. In this study, we attempted to engineer the titanium (Ti) alloy surface biomimetically at the nanometer scale, inspired by the cicada wing nanostructure using alkaline hydrothermal treatment (AHT) to simultaneously confer antibacterial properties and support the adhesion and proliferation of mammalian cells. The two modified Ti surfaces were developed using a 4 h and 8 h AHT process in 1 N NaOH at 230 °C, followed by a 2-hour post-calcination at 600 °C. We found that the control plates showed a relatively smooth surface, while the treatment groups (4 h & 8 h AHT) displayed nanoflower structures containing randomly distributed nano-spikes. The results demonstrated a statistically significant decrease in the contact angle of the treatment groups, which increased wettability characteristics. The 8 h AHT group exhibited the highest wettability and significant increase in roughness 0.72 ± 0.08 µm (P < 0.05), leading to more osteoblast cell attachment, reduced cytotoxicity effects, and enhanced relative survivability. The alkaline phosphatase activity measured in all different groups indicated that the 8 h AHT group exhibited the highest activity, suggesting that the surface roughness and wettability of the treatment groups may have facilitated cell adhesion and attachment and subsequently increased secretion of extracellular matrix. Overall, the findings indicate that biomimetic nanotextured surfaces created by the AHT process have the potential to be translated as implant coatings to enhance bone regeneration and implant integration.
期刊介绍：
The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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