Hans Hebert , Elisabeth Skriver , Reiner Hegerl , Arvid B. Maunsbach
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引用次数: 10
摘要
用相关平均法分析了兔肾中膜结合Na, k - atp酶的二维晶体结构。两种主要不同的晶体形式分别观察到p1和p21对称性。在p1型中,平均投影结构显示一个三角形的蛋白质结构域,解释为Na, k - atp酶的原聚体(αβ-单位)。在p21型中,染色缺陷区向双对称轴方向延伸。结果与先前的傅里叶方法应用于有序的猪肾Na, k - atp酶晶体的分析结果很好地一致,并说明相关平均程序可用于分析Na, k - atp酶的膜晶体,显示弯曲的晶格线。
Structure of two-dimensional crystals of membrane-bound Na,K-ATPase as analyzed by correlation averaging
The structure of two-dimensional crystals of membrane-bound Na,K-ATPase from rabbit kidney has been analyzed with a correlation averaging procedure. Two principally different crystal forms are observed with p1 and p21 symmetry, respectively. In the p1 form the averaged projection structure shows a triangular shaped protein domain interpreted as a protomer (αβ-unit) of Na,K-ATPase. In the p21-form the stain-deficient area is extended toward a twofold symmetry axis. The results are in good agreement with a previous analysis where Fourier methods were applied to well ordered crystals of pig kidney Na,K-ATPase and illustrate that the correlation averaging procedure can be used for the analysis of membrane crystals of Na,K-ATPase showing curved lattice lines.