{"title":"比较三种不同的碳水化合物体外消化方法","authors":"Yahao Xiao, Sheng Li, Jiaxi Li, Yanlan Bi, Xuebing Xu, Hong Zhang","doi":"10.1007/s13197-024-06012-1","DOIUrl":null,"url":null,"abstract":"<div><p>Spectrophotometer method, ELISA, and High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) method have been widely used to quantify and characterize the glucose released from rice after in vitro digestion. Despite this, the results of the three methods may not be comparable. This work investigated the limitation of detection (LOD) and quantification (LOQ) of the glucose released after in vitro rice digestion. Intra-day and inter-day precision were evaluated through analyzing the contents of rapidly digestible starch (RDS), DS (RDS + slowly digestible starch (SDS)), and total starch (TS). Tests of accuracies were also conducted. The results demonstrated that all three methods showed good linear correlations, i.e., y = 0.3417x + 0.0799 (r<sup>2</sup> = 0.994), y = 0.1719x + 0.0900 (r<sup>2</sup> = 0.993), and y = 58078x + 6039.4 (r<sup>2</sup> = 0.999), respectively. Concerning the LOD and LOQ, HPAEC-PAD showed the lowest 0.07 and 0.24 mg/L, followed by the Spectrophotometer of 2.72 and 9.07 mg/L. ELISA showed the highest 9.95 and 33.16 mg/L. The recoveries of these methods ranged from 98.62% to 100.56%, 99.05% to 102.72%, and 96.71% to 103.27%, respectively. HPAEC-PAD method had lower contents of RDS, DS, and TS, indicating it may be poor. Combining above factors, Spectrophotometer was chosen and used in digestion of biscuits and steamed buns. RS of biscuits was the highest, possibly due to the formation of lipid-starch complex. Additionally, steamed buns had the highest RDS. These observations suggested that Spectrophotometer method was suitable for accurately and reproducibly analyzing carbohydrate digestion in different food systems.</p></div>","PeriodicalId":632,"journal":{"name":"Journal of Food Science and Technology","volume":"62 1","pages":"88 - 97"},"PeriodicalIF":2.7010,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of three different in vitro digestion methods for carbohydrates\",\"authors\":\"Yahao Xiao, Sheng Li, Jiaxi Li, Yanlan Bi, Xuebing Xu, Hong Zhang\",\"doi\":\"10.1007/s13197-024-06012-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Spectrophotometer method, ELISA, and High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) method have been widely used to quantify and characterize the glucose released from rice after in vitro digestion. Despite this, the results of the three methods may not be comparable. This work investigated the limitation of detection (LOD) and quantification (LOQ) of the glucose released after in vitro rice digestion. Intra-day and inter-day precision were evaluated through analyzing the contents of rapidly digestible starch (RDS), DS (RDS + slowly digestible starch (SDS)), and total starch (TS). Tests of accuracies were also conducted. The results demonstrated that all three methods showed good linear correlations, i.e., y = 0.3417x + 0.0799 (r<sup>2</sup> = 0.994), y = 0.1719x + 0.0900 (r<sup>2</sup> = 0.993), and y = 58078x + 6039.4 (r<sup>2</sup> = 0.999), respectively. Concerning the LOD and LOQ, HPAEC-PAD showed the lowest 0.07 and 0.24 mg/L, followed by the Spectrophotometer of 2.72 and 9.07 mg/L. ELISA showed the highest 9.95 and 33.16 mg/L. The recoveries of these methods ranged from 98.62% to 100.56%, 99.05% to 102.72%, and 96.71% to 103.27%, respectively. HPAEC-PAD method had lower contents of RDS, DS, and TS, indicating it may be poor. Combining above factors, Spectrophotometer was chosen and used in digestion of biscuits and steamed buns. RS of biscuits was the highest, possibly due to the formation of lipid-starch complex. Additionally, steamed buns had the highest RDS. These observations suggested that Spectrophotometer method was suitable for accurately and reproducibly analyzing carbohydrate digestion in different food systems.</p></div>\",\"PeriodicalId\":632,\"journal\":{\"name\":\"Journal of Food Science and Technology\",\"volume\":\"62 1\",\"pages\":\"88 - 97\"},\"PeriodicalIF\":2.7010,\"publicationDate\":\"2024-06-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Food Science and Technology\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13197-024-06012-1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Science and Technology","FirstCategoryId":"1","ListUrlMain":"https://link.springer.com/article/10.1007/s13197-024-06012-1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Comparison of three different in vitro digestion methods for carbohydrates
Spectrophotometer method, ELISA, and High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) method have been widely used to quantify and characterize the glucose released from rice after in vitro digestion. Despite this, the results of the three methods may not be comparable. This work investigated the limitation of detection (LOD) and quantification (LOQ) of the glucose released after in vitro rice digestion. Intra-day and inter-day precision were evaluated through analyzing the contents of rapidly digestible starch (RDS), DS (RDS + slowly digestible starch (SDS)), and total starch (TS). Tests of accuracies were also conducted. The results demonstrated that all three methods showed good linear correlations, i.e., y = 0.3417x + 0.0799 (r2 = 0.994), y = 0.1719x + 0.0900 (r2 = 0.993), and y = 58078x + 6039.4 (r2 = 0.999), respectively. Concerning the LOD and LOQ, HPAEC-PAD showed the lowest 0.07 and 0.24 mg/L, followed by the Spectrophotometer of 2.72 and 9.07 mg/L. ELISA showed the highest 9.95 and 33.16 mg/L. The recoveries of these methods ranged from 98.62% to 100.56%, 99.05% to 102.72%, and 96.71% to 103.27%, respectively. HPAEC-PAD method had lower contents of RDS, DS, and TS, indicating it may be poor. Combining above factors, Spectrophotometer was chosen and used in digestion of biscuits and steamed buns. RS of biscuits was the highest, possibly due to the formation of lipid-starch complex. Additionally, steamed buns had the highest RDS. These observations suggested that Spectrophotometer method was suitable for accurately and reproducibly analyzing carbohydrate digestion in different food systems.
期刊介绍:
The Journal of Food Science and Technology (JFST) is the official publication of the Association of Food Scientists and Technologists of India (AFSTI). This monthly publishes peer-reviewed research papers and reviews in all branches of science, technology, packaging and engineering of foods and food products. Special emphasis is given to fundamental and applied research findings that have potential for enhancing product quality, extend shelf life of fresh and processed food products and improve process efficiency. Critical reviews on new perspectives in food handling and processing, innovative and emerging technologies and trends and future research in food products and food industry byproducts are also welcome. The journal also publishes book reviews relevant to all aspects of food science, technology and engineering.