Litong Zhu , Yick Hei Wong , Sunny S.H. Wong , Simon C.Y. Cheung , Jason K.H. Sher , Irene Y.L. Yam , Susan Yung , Tak Mao Chan , Desmond Y.H. Yap
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The expression of inhibitory/adhesion molecules, cell proliferation and calcium mobilization in classical memory and exhausted B cells were also assessed.</p></div><div><h3>Results</h3><p>The MR group had higher proportion of circulating exhausted and classical memory B cells compared to the NR group and healthy controls (HC) (p all <0.05 for MR vs. NR or HC). Blood levels of IL-6, BAFF, IL-21, CD62L, CXCR3 and Siglec-6 were all higher in the MR group (<em>p</em> < 0.05, for all). Exhausted B cells from the MR group showed higher FcRL4, CD22, CD85j and CD183 but lower CD62L expression than NR and HC groups. Exhausted B cells from MR patients exhibited reduced proliferation compared to NR patients and HC, while classical memory B cell proliferation in MR group was higher than the other two groups. Exhausted B cells from both MR and NR patients showed impaired calcium mobilization.</p></div><div><h3>Conclusion</h3><p>Alterations in exhausted and classical memory B cells are related to disease relapse in LN. These findings may help devise new strategies for monitoring disease activity and preventing relapse in LN.</p></div>","PeriodicalId":10392,"journal":{"name":"Clinical immunology","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1521661624003930/pdfft?md5=11b0e15f4ee4915608200ebb30e145ec&pid=1-s2.0-S1521661624003930-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Alterations in exhausted and classical memory B cells in lupus nephritis – Relationship with disease relapse\",\"authors\":\"Litong Zhu , Yick Hei Wong , Sunny S.H. Wong , Simon C.Y. Cheung , Jason K.H. Sher , Irene Y.L. Yam , Susan Yung , Tak Mao Chan , Desmond Y.H. Yap\",\"doi\":\"10.1016/j.clim.2024.110284\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>B cell exhaustion is a functional abnormality of B lymphocytes observed in chronic infections and shows association with autoreactivity. The role of exhausted and classical memory B cells in maintaining disease stability of lupus nephritis (LN) remains unclear.</p></div><div><h3>Methods</h3><p>We measured classical memory (CD19<sup>+</sup>CD21<sup>+</sup>CD27<sup>+</sup>), exhausted B cells (CD19<sup>+</sup>CD21<sup>−</sup>CD27<sup>−</sup>), and related cytokines in LN patients with multiple relapses (MR) (<em>n</em> = 15) and no relapse (NR) (n = 15) during disease remission. The expression of inhibitory/adhesion molecules, cell proliferation and calcium mobilization in classical memory and exhausted B cells were also assessed.</p></div><div><h3>Results</h3><p>The MR group had higher proportion of circulating exhausted and classical memory B cells compared to the NR group and healthy controls (HC) (p all <0.05 for MR vs. NR or HC). Blood levels of IL-6, BAFF, IL-21, CD62L, CXCR3 and Siglec-6 were all higher in the MR group (<em>p</em> < 0.05, for all). Exhausted B cells from the MR group showed higher FcRL4, CD22, CD85j and CD183 but lower CD62L expression than NR and HC groups. Exhausted B cells from MR patients exhibited reduced proliferation compared to NR patients and HC, while classical memory B cell proliferation in MR group was higher than the other two groups. Exhausted B cells from both MR and NR patients showed impaired calcium mobilization.</p></div><div><h3>Conclusion</h3><p>Alterations in exhausted and classical memory B cells are related to disease relapse in LN. 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引用次数: 0
摘要
引言B细胞衰竭是慢性感染时观察到的一种B淋巴细胞功能异常,与自身反应性有关。衰竭B细胞和经典记忆B细胞在维持狼疮性肾炎(LN)疾病稳定性方面的作用仍不清楚:我们测量了疾病缓解期间多次复发(MR)(15 人)和未复发(NR)(15 人)的狼疮肾炎患者的经典记忆 B 细胞(CD19+CD21+CD27+)、衰竭 B 细胞(CD19+CD21-CD27-)和相关细胞因子。此外,还评估了经典记忆B细胞和衰竭B细胞中抑制/粘附分子的表达、细胞增殖和钙动员情况:结果:与 NR 组和健康对照组(HC)相比,MR 组的循环衰竭和经典记忆 B 细胞比例较高(P 均为结论:衰竭和经典记忆 B 细胞的变化与 LN 疾病复发有关。这些发现可能有助于制定监测 LN 疾病活动和预防复发的新策略。
Alterations in exhausted and classical memory B cells in lupus nephritis – Relationship with disease relapse
Introduction
B cell exhaustion is a functional abnormality of B lymphocytes observed in chronic infections and shows association with autoreactivity. The role of exhausted and classical memory B cells in maintaining disease stability of lupus nephritis (LN) remains unclear.
Methods
We measured classical memory (CD19+CD21+CD27+), exhausted B cells (CD19+CD21−CD27−), and related cytokines in LN patients with multiple relapses (MR) (n = 15) and no relapse (NR) (n = 15) during disease remission. The expression of inhibitory/adhesion molecules, cell proliferation and calcium mobilization in classical memory and exhausted B cells were also assessed.
Results
The MR group had higher proportion of circulating exhausted and classical memory B cells compared to the NR group and healthy controls (HC) (p all <0.05 for MR vs. NR or HC). Blood levels of IL-6, BAFF, IL-21, CD62L, CXCR3 and Siglec-6 were all higher in the MR group (p < 0.05, for all). Exhausted B cells from the MR group showed higher FcRL4, CD22, CD85j and CD183 but lower CD62L expression than NR and HC groups. Exhausted B cells from MR patients exhibited reduced proliferation compared to NR patients and HC, while classical memory B cell proliferation in MR group was higher than the other two groups. Exhausted B cells from both MR and NR patients showed impaired calcium mobilization.
Conclusion
Alterations in exhausted and classical memory B cells are related to disease relapse in LN. These findings may help devise new strategies for monitoring disease activity and preventing relapse in LN.
期刊介绍:
Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.