桑叶中的 24(R)-ethyllophenol 对 PC12 细胞神经元生长的促进作用

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Pharmazie Pub Date : 2024-05-15 DOI:10.1691/ph.2024.3656

S Honma, K Handa, R Mitsumata, D Shimada, S Takei, G Tanaka, K Watanabe, M Yoshida

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引用次数: 0

摘要

我们研究了从桑树枝条中分离出的24(R)-乙基苯酚（MAB28）引起大鼠嗜铬细胞瘤细胞（PC12）神经元生长的机制。MAB28 对神经元生长的促进作用与阳性对照神经生长因子（NGF）相似。PC12 细胞与 MAB28 培养后，检测到细胞外信号调节激酶、p38 丝裂原活化蛋白激酶和环 AMP 反应元件结合蛋白磷酸化，但磷酸化的时间过程与 NGF 诱导的不同。MAB28 显著降低了氯离子胞内通道蛋白 3（CLIC3）的表达。5-硝基-2-（3-苯基丙基氨基）苯甲酸（NPPB）是一种外向整流氯离子通道抑制剂，它能显著促进PC12细胞神经元的生长。这些数据表明，MAB28 可通过下调 CLIC3 的表达来诱导神经元生长。

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Neurite outgrowth promotion in PC12 cells by 24(R)-ethyllophenol from Morus alba.

We examined the mechanism by which 24(R)-ethyllophenol (MAB28) isolated from the branches of Morus alba caused neurite outgrowth in rat pheochromocytoma cells (PC12). MAB28 significantly promoted neurite outgrowth to a similar degree as the positive control, nerve growth factor (NGF). After incubation with MAB28 in PC12 cells, phosphorylation of extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and cyclic AMP response element-binding protein was detected, but the time course of phosphorylation was different from that induced by NGF. The expression of chloride intracellular channel protein 3 (CLIC3) was significantly decreased by MAB28. 5-Nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), an outward rectifying chloride channel inhibitor, significantly promoted neurite outgrowth in PC12 cells. These data suggested that MAB28 could induce neurite outgrowth by downregulating CLIC3 expression.

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来源期刊

Pharmazie 医学-化学综合

CiteScore

3.10

自引率

0.00%

发文量

审稿时长

1.2 months

期刊介绍： The journal DiePharmazie publishs reviews, experimental studies, letters to the editor, as well as book reviews. The following fields of pharmacy are covered: Pharmaceutical and medicinal chemistry; Pharmaceutical analysis and drug control; Pharmaceutical technolgy; Biopharmacy (biopharmaceutics, pharmacokinetics, biotransformation); Experimental and clinical pharmacology; Pharmaceutical biology (pharmacognosy); Clinical pharmacy; History of pharmacy.