在商用质谱仪上实施纳米喷雾解吸电喷雾离子源(nano-DESI)的硬件和软件解决方案。

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS
Li-Xue Jiang, Ryan T. Hilger, Julia Laskin
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引用次数: 0

摘要

纳米喷雾解吸电喷雾离子化(nano-DESI)是一种环境电离质谱成像(MSI)方法,能以高空间分辨率和高通量绘制生物和环境样品的空间图谱。由于纳米电离质谱成像尚未商业化,研究人员需要开发自己的源,并将其与不同的商业质谱仪连接。在此之前,已经有几篇关于纳米 DESI 探针制作方法的报道。在本教程中,我们将讨论将纳米 DESI 源与商用质谱仪连接的不同硬件要求,如安全联锁、入口扩展和接触闭合。此外,我们还介绍了用于控制纳米-DESI MSI 平台的定制软件的结构,并提供了详细的使用说明。通过本教程,感兴趣的研究人员应该能够在自己的实验室中实施纳米-DESI 实验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Hardware and software solutions for implementing nanospray desorption electrospray ionization (nano-DESI) sources on commercial mass spectrometers

Hardware and software solutions for implementing nanospray desorption electrospray ionization (nano-DESI) sources on commercial mass spectrometers

Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization mass spectrometry imaging (MSI) approach that enables spatial mapping of biological and environmental samples with high spatial resolution and throughput. Because nano-DESI has not yet been commercialized, researchers develop their own sources and interface them with different commercial mass spectrometers. Previously, several protocols focusing on the fabrication of nano-DESI probes have been reported. In this tutorial, we discuss different hardware requirements for coupling the nano-DESI source to commercial mass spectrometers, such as the safety interlock, inlet extension, and contact closure. In addition, we describe the structure of our custom software for controlling the nano-DESI MSI platform and provide detailed instructions for its usage. With this tutorial, interested researchers should be able to implement nano-DESI experiments in their labs.

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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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