向绵羊黄体注入干扰素可延缓黄体溶解。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Aydin Guzeloglu, Jeanette V Bishop, Hana Van Campen, Michele R Plewes, Carolina L Gonzalez-Berrios, Jessica N Kincade, John S Davis, Thomas R Hansen
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引用次数: 0

摘要

来源于受体的干扰素-tau(IFNT)通过对子宫内膜的旁分泌作用和对黄体(CL)的内分泌作用启动母羊对妊娠的识别。为了研究 IFNT 在不诱导子宫内膜 IFN 刺激基因(ISGs)的情况下对 CL 的影响,在发情周期的第 9 到 12 天,通过渗透泵以 10、50 或 100 纳克/小时的速度将重组绵羊 IFNT(roIFNT)或牛血清白蛋白(BSA)直接输送到 CL。第 12 天采集子宫内膜和 CL 样本。在第 12 天,50 纳克/小时的 roIFNT 会诱导 CL 中的 ISG15,但不会影响子宫内膜 ISG15 的浓度。在第二个实验中,从发情周期的第 10 天到第 17 天将 roIFNT(50 纳克/小时)注入 CL,并每天收集血清样本。与对照组相比,输注 roIFNT 的母羊在第 15 至 17 天的血清孕酮浓度明显更高。与对照组相比,注射了 roIFNT 的 CL 中 LHCGR、STAR、CYP11A1、HSL、OPA1 和 PKA mRNA 和蛋白质的水平较高。在注射了 roIFNT 的母羊的 CL 中,ISG15 和 MX1 mRNA 的水平升高,但在子宫内膜中却没有升高。与注射了 roIFNT 的母羊相比,对照组的子宫内膜 ESR1 mRNA 和蛋白质浓度更高。总之,黄体内注射 roIFNT 可诱导 ISGs、稳定 CL 中的类固醇生成并延迟黄体溶解,但不会诱导子宫内膜 ISGs。在注射了roIFNT的母羊子宫内膜中观察到了ESR1的抑制作用,这表明直接将IFNT输送到CL对子宫内膜具有额外的抗黄体溶解作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interferon-tau infusion into the ovine corpus luteum delays luteolysis†.

Conceptus-derived interferon-tau (IFNT) initiates maternal recognition of pregnancy in ewes by paracrine actions on the endometrium and endocrine action on the corpus luteum (CL). To examine the effect of IFNT on the CL without inducing IFN-stimulated genes (ISGs) in the endometrium, recombinant ovine IFNT (roIFNT) or bovine serum albumin was delivered directly into CLs via osmotic pumps at a rate of 10, 50, or 100 ng/h from days 9 to 12 of the estrous cycle. Endometrial and CL samples were collected on day 12. 50 ng/h of roIFNT induced ISG15 in the CL on day 12 without affecting endometrial ISG15 concentrations. In a second experiment, roIFNT (50 ng/h) was infused into the CL from days 10 to 17 of the estrous cycle and serum samples were collected daily. Serum progesterone concentrations were significantly higher from days 15 to 17 in roIFNT-infused ewes compared to controls. Levels of LHCGR, STAR, CYP11A1, HSL, OPA1, and protein kinase A mRNA and proteins were higher in the roIFNT-infused CLs compared to the controls. Levels of ISG15 and MX1 mRNA increased in the CLs of roIFNT-infused ewes but not in the endometrium. Endometrial ESR1 mRNA and protein concentrations were higher in the controls compared to roIFNT-infused ewes. In conclusion, intra-luteal delivery of roIFNT induced ISGs, stabilized steroidogenesis in the CL, and delayed luteolysis without inducing endometrial IFN-stimulated genes. Inhibition of ESR1 in the endometrium of roIFNT-infused ewes was observed suggesting that direct delivery of IFNT to the CL has an additional anti-luteolytic effect on the endometrium.

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CiteScore
7.20
自引率
4.30%
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567
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