Jijuan Zhou, Dianlong Ge*, Yajing Chu, Yue Liu, Yan Lu and Yannan Chu*,
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引用次数: 0
摘要
检测呼出的挥发性有机化合物(VOC)有望用于食管癌(EC)的无创筛查。细胞挥发性有机化合物分析可用于研究潜在的生物标记物。考虑到蛋氨酸(Met)在癌症发展过程中的关键作用,探索相关的异常代谢表型势在必行。在这项研究中,我们采用顶空固相微萃取-气相色谱-质谱联用仪(HS-SPME-GC-MS)研究了Met调控策略下EC细胞(KYSE150)和正常食管上皮细胞(HEECs)的挥发性代谢谱。我们使用非靶向方法分析了两种细胞类型的代谢挥发性有机化合物,并探索了它们之间的挥发性有机化合物差异。随后,我们利用靶向方法分析了两种细胞在梯度 Met 培养条件下的不同 VOCs。结果表明,在含金属元素/不含金属元素的培养条件下,细胞间存在五种/六种不同的挥发性有机化合物。而随着 Met 浓度的增加,两种细胞间两种特征性挥发性有机化合物(1-丁醇和 2-甲基丁酸乙酯)的含量差异也在加剧。值得注意的是,这是第一份关于欧共体细胞挥发性有机化合物分析的报告,也是第一份考虑 Met 对挥发性代谢特征影响的报告。本研究结果表明,可以通过 Met 调节诱导的挥发性有机化合物来区分心肌细胞,这有望为诊断策略提供新的见解。
Distinguish Esophageal Cancer Cells through VOCs Induced by Methionine Regulation
Detection of exhaled volatile organic compounds (VOCs) is promising for noninvasive screening of esophageal cancer (EC). Cellular VOC analysis can be used to investigate potential biomarkers. Considering the crucial role of methionine (Met) during cancer development, exploring associated abnormal metabolic phenotypes becomes imperative. In this work, we employed headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) to investigate the volatile metabolic profiles of EC cells (KYSE150) and normal esophageal epithelial cells (HEECs) under a Met regulation strategy. Using untargeted approaches, we analyzed the metabolic VOCs of the two cell types and explored the differential VOCs between them. Subsequently, we utilized targeted approaches to analyze the differential VOCs in both cell types under gradient Met culture conditions. The results revealed that there were five/six differential VOCs between cells under Met-containing/Met-free culture conditions. And the difference in levels of two characteristic VOCs (1-butanol and ethyl 2-methylbutyrate) between the two cell types intensified with the increase of the Met concentration. Notably, this is the first report on VOC analysis of EC cells and the first to consider the effect of Met on volatile metabolic profiles. The present work indicates that EC cells can be distinguished through VOCs induced by Met regulation, which holds promise for providing novel insights into diagnostic strategies.
期刊介绍:
Journal of Proteome Research publishes content encompassing all aspects of global protein analysis and function, including the dynamic aspects of genomics, spatio-temporal proteomics, metabonomics and metabolomics, clinical and agricultural proteomics, as well as advances in methodology including bioinformatics. The theme and emphasis is on a multidisciplinary approach to the life sciences through the synergy between the different types of "omics".