车辆和灌溉溶液及方法对根管中双抗药膏去除效果的影响:高效液相色谱分析的启示。

IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
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引用次数: 0

摘要

导言:在牙髓再生治疗中,鉴于抗生素对人类根尖乳头干细胞的有害影响,清除根管内的抗生素残留物势在必行。以往的抗生素清除研究无法精确确定残留抗生素的类型和数量。高效液相色谱法(HPLC)提高了灵敏度和特异性,能够准确检测和量化残留药物。本研究利用高效液相色谱分析,探讨了载体、冲洗溶液和方法对根管中双抗生素糊剂(DAP)去除的影响:使用不同的载体:大环内酯和丙二醇(MP)或羟丙基甲基纤维素(HPMC)配制了两种 DAP 配方,每种配方均含有 5 mg/mL 环丙沙星和甲硝唑。随后,将 5 μL 的 DAP 涂在 200 颗尖端开放的模拟未成熟牙齿上(每种配方 100 颗),并在 37°C 下培养 28 天。然后将样本分为 11 组(每组 n = 20),采用正压注射器或被动超声波灌洗法,用 17% 乙二胺四乙酸或 10% 柠檬酸灌洗牙根管。通过高效液相色谱法对每颗牙齿的灌洗液和牙本质样本进行环丙沙星和甲硝唑定量评估:结果:柠檬酸对根管中抗生素的去除效果明显优于其他抗生素,灌洗方法对药物去除效果没有明显影响。与基于 MP 的 DAP 相比,基于 HPMC 的 DAP 配方能显著提高环丙沙星的去除率:结论:柠檬酸能有效去除根管中的抗生素糊剂,HPMC是比MP更理想的载体。总之,高效液相色谱法是检测、去除和量化根管内残留抗生素的重要方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Vehicles and Irrigation Solutions and Methods on Removal of Double Antibiotic Paste from Root Canals: Insights from High-performance Liquid Chromatography Analysis

Introduction

In regenerative endodontics, eradicating antibiotic residues from root canals is imperative, given their detrimental effects on human apical papilla stem cells. Previous antibiotic removal studies lacked precision in identifying types and quantities of residual antibiotics. High-performance liquid chromatography (HPLC) enhances sensitivity and specificity, enabling accurate detection and quantification of residual drugs. Using HPLC analysis, this study explored the influence of vehicles and irrigation solutions and methods on double antibiotic paste (DAP) removal from root canals.

Methods

Two DAP formulations, each containing 5 mg/mL ciprofloxacin and metronidazole, were created using distinct vehicles: macrogol and propylene glycol (MP) or hydroxypropyl methylcellulose (HPMC). Subsequently, 5 μL of DAP was applied to 200 simulated immature teeth with open apices (n = 100 per formulation) and cultured for 28 days at 37°C. Samples were then divided into 11 groups (n = 20 per group), and canals were irrigated with 17% ethylenediaminetetraacetic acid or 10% citric acid, employing a positive pressure syringe or passive ultrasonic irrigation. The irrigation solution and dentin sample from each tooth were evaluated via HPLC for ciprofloxacin and metronidazole quantification.

Results

Citric acid exhibited significantly superior efficacy in antibiotic removal from root canals, with no observable effect of irrigation methods on drug removal. The HPMC-based DAP formulation significantly enhanced ciprofloxacin removal compared with MP-based DAP.

Conclusions

For antibiotic paste removal from root canals, citric acid is effective, and HPMC is a preferable vehicle over MP. Overall, HPLC is a valuable method for detecting, removing, and quantifying residual antibiotics in root canals.

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来源期刊
Journal of endodontics
Journal of endodontics 医学-牙科与口腔外科
CiteScore
8.80
自引率
9.50%
发文量
224
审稿时长
42 days
期刊介绍: The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.
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