日本海参口周神经环的神经细胞群:超微结构和转录特征

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Yingqiu Zheng , Xiao Cong , Huachen Liu , Kenneth B. Storey , Muyan Chen
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引用次数: 0

摘要

棘皮动物的神经系统是了解脊索动物神经系统进化的模型。神经元细胞是神经系统中释放 "鸡尾酒 "信使分子的重要群体,可提供一系列生物作用。在棘皮动物中,有关神经元细胞类型的证据大多来自海星和海胆。在海参中,大多数研究集中于神经元细胞的位置,而对其转录特征的研究则很少。在这里,我们观察了海参神经元细胞的超微结构。利用单细胞 RNA 测序(scRNA-seq)研究了口周神经环(CNR)神经细胞的转录特征,共鉴定出六种神经细胞类型。在这六种神经细胞类型中表达了 26 个神经肽前体基因(NPPs)和 28 个 G 蛋白偶联受体(GPCR),其中包括五对 NPP/NP-GPCR 对。神经元细胞的无监督伪时间分析显示了它们的不同分化状态。我们还通过免疫荧光(IF)定位了 CNR 中的神经元细胞,并确定了关键细胞群的潜在枢纽基因。这一广泛的资源为开发细胞特异性标记物提供了宝贵的支持,有助于准确鉴定海参的细胞类型。它还有助于促进物种间的比较,加深对神经元细胞进化过程的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Neuronal cell populations in circumoral nerve ring of sea cucumber Apostichopus japonicus: Ultrastructure and transcriptional profile

Neuronal cell populations in circumoral nerve ring of sea cucumber Apostichopus japonicus: Ultrastructure and transcriptional profile

The echinoderm nervous system has been studied as a model for understanding the evolution of the chordate nervous system. Neuronal cells are essential groups that release a ‘cocktail’ of messenger molecules providing a spectrum of biological actions in the nervous system. Among echinoderms, most evidence on neuronal cell types has been obtained from starfish and sea urchin. In sea cucumbers, most research has focused on the location of neuronal cells, whereas their transcriptional features have rarely been investigated. Here, we observed the ultrastructure of neuronal cells in the sea cucumber, Apostichopus japonicus. The transcriptional profile of neuronal cells from the circumoral nerve ring (CNR) was investigated using single-cell RNA sequencing (scRNA-seq), and a total of six neuronal cell types were identified. 26 neuropeptide precursor genes (NPPs) and 28 G-protein-coupled receptors (GPCR) were expressed in the six neuronal cell types, comprising five NPP/NP-GPCR pairs. Unsupervised pseudotime analysis of neuronal cells showed their different differentiation status. We also located the neuronal cells in the CNR by immunofluorescence (IF) and identified the potential hub genes of key cell populations. This broad resource serves as a valuable support in the development of cell-specific markers for accurate cell-type identification in sea cucumbers. It also contributes to facilitating comparison across species, providing a deeper understanding of the evolutionary processes of neuronal cells.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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