支气管刷洗、活检前和活检后灌洗细胞学中细胞产量的数字图像比较。

IF 1.6 4区 医学 Q3 PATHOLOGY
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-06-05 DOI:10.1159/000539567
Joshua Jing Xi Li, Joanna Ka Man Ng, Christopher Chan, Charlotte Ho Ying Lau, Joyce Ka Ching Ng, Rachel Lai Ping Lo, Wing Ho Yip, Jenny Chun Li Ngai, Ka Pang Chan
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引用次数: 0

摘要

背景 支气管镜是一种有用的诊断工具,能够进行核心活检、镊子活检、支气管肺泡灌洗和支气管刷洗。本研究通过数字图像分析比较支气管细胞学检查(包括活检前和活检后灌洗)的细胞学特性,旨在通过优化支气管活检和细胞学检查的顺序和组合,提高诊断率和肿瘤检出率。方法 将支气管刷洗、活检前和活检后支气管肺泡灌洗的液基细胞学制备物中的肺泡巨噬细胞、支气管上皮细胞和肿瘤细胞标注在数字化全切片图像上并进行比较。对肿瘤细胞和非病变细胞产量的关系进行了二次分析。结果 共检索到 43 名患者的 118 份细胞学标本。活组织检查前的支气管上皮细胞数高于活组织检查后的灌洗细胞数(p<0.01),但肺泡巨噬细胞和肿瘤细胞数则不高(p>0.05)。支气管刷状细胞学样本的肿瘤细胞计数高于灌洗样本(p=0.018)。活检后灌洗样本的肺泡巨噬细胞计数高于支气管刷检样本(p=0.033),否则刷检样本的支气管上皮细胞和肿瘤细胞计数一直较高。有 33 例假阴性(无肿瘤细胞)标本,支气管刷检和活检前灌洗的组合产生的假阴性病例最少。支气管上皮细胞和肺泡巨噬细胞计数与肿瘤细胞计数之间的相关性较弱(相关系数=-0.168-0.203),但活检后灌洗除外(相关系数=0.412-0.479,p<0.05)。结论 支气管刷洗比灌洗获得更多的肿瘤细胞,在核心活检之前或之后进行灌洗不会影响肿瘤细胞的数量。结合支气管刷洗和活检前灌洗可使假阴性率降到最低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Digital Image Comparison of Cellular Yield in Bronchial Brushing: Pre- and Post-Biopsy Lavage Cytology.

Introduction: Bronchoscopy is a useful diagnostic tool capable of performing core biopsy, forceps biopsy, bronchoalveolar lavage, and bronchial brushing. This study compares the cellularity of bronchial cytology including pre- and post-biopsy lavage by digital image analysis, aiming to increase diagnostic and tumor yield by optimizing the sequence and combination of bronchial biopsy and cytology.

Methods: Alveolar macrophage, bronchial epithelium, and tumor cell cellularity from liquid-based cytology preparations of bronchial brushing and pre-biopsy and post-biopsy bronchoalveolar lavage were annotated on digitized whole-slide images and compared. Secondary analysis on the relationship of tumor cell and non-lesional cell yield was performed.

Results: Overall, 118 cytology specimens from 43 patients were retrieved in total. Bronchial epithelium count was higher in pre-biopsy than post-biopsy lavage (p < 0.01) but not for alveolar macrophages nor tumor cell (p > 0.05). Tumor cell count was higher for bronchial brushing cytology samples than lavage (p = 0.018). The alveolar macrophage count was higher in post-biopsy lavage than bronchial brushing (p = 0.033); otherwise, brushing showed consistently higher bronchial epithelium and tumor cell counts. There were 33 false negative (tumor cell absent) specimens, and the combination of bronchial brushing and pre-biopsy lavage yielded the lowest false negative cases. Correlation between bronchial epithelium and alveolar macrophage counts with tumor cell count was weak (correlation coefficient = -0.168-0.203) except for post-biopsy lavage (correlation coefficient = 0.412-0.479, p < 0.05).

Conclusion: Bronchial brushing yields a greater amount of tumor cell than lavage, and timing lavage before or after core biopsy does not affect tumor cell yield. Combining bronchial brushing and pre-biopsy lavage results in the lowest false negative rate.

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来源期刊
Acta Cytologica
Acta Cytologica 生物-病理学
CiteScore
3.70
自引率
11.10%
发文量
46
审稿时长
4-8 weeks
期刊介绍: With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.
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