脂肪素原和网膜素能直接调节猫卵巢颗粒细胞的功能

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2024-05-31 DOI:10.1016/j.rvsc.2024.105321

Alexander V. Sirotkin , Zuzana Fabová , Barbora Loncová , Maria Bauerová , Abdel Halim Harrath

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引用次数: 0

摘要

本研究的目的是确定脂肪因子原花青素和网织红蛋白对猫卵巢细胞基本功能的影响。为此，我们通过定量免疫细胞化学和酶联免疫吸附试验（ELISA），研究了添加前胡素和网织红蛋白（0、0.1、1或10纳克/毫升）对培养的猫卵巢颗粒细胞的增殖（PCNA和细胞周期蛋白B1的积累）、凋亡（Bax和caspase 3的积累）和孕酮释放的影响。孕酮素和网织红素都能增加细胞增殖，减少细胞凋亡。原胰岛素和网织红蛋白都能促进孕酮的释放。本研究结果表明，脂肪因子原促胰岛素和网织红蛋白可直接调节猫卵巢细胞的基本功能。

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The adipokines progranulin and omentin can directly regulate feline ovarian granulosa cell functions

The aim of the present study was to determine the effects of the adipokines progranulin and omentin on the basic functions of feline ovarian cells. For this purpose, we investigated the effects of the addition of progranulin and omentin (0, 0.1, 1, or 10 ng/ml) on the proliferation (accumulation of PCNA and cyclin B1), apoptosis (accumulation of Bax and caspase 3) and progesterone release of cultured feline ovarian granulosa cells by quantitative immunocytochemistry and enzyme-linked immunosorbent assays (ELISAs). Both progranulin and omentin increased cell proliferation and decreased apoptosis. Both progranulin and omentin promoted progesterone release. The present findings demonstrate that the adipokines progranulin and omentin can directly regulate basic feline ovarian cell functions.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.