Yuping Xiong , Xiaohong Chen , Junyu Liu , Jianrong Li , Zhan Bian , Yuan Li , Xinhua Zhang , Songjun Zeng , Guohua Ma
{"title":"试管繁殖过程中黑暗诱导 Euryodendron excelsum H. T. Chang 不定根的转录组和激素综合分析","authors":"Yuping Xiong , Xiaohong Chen , Junyu Liu , Jianrong Li , Zhan Bian , Yuan Li , Xinhua Zhang , Songjun Zeng , Guohua Ma","doi":"10.1016/j.cpb.2024.100353","DOIUrl":null,"url":null,"abstract":"<div><p><em>Euryodendron excelsum</em> H. T. Chang, a rare and endangered evergreen tree that is endemic to China. The micropropagation system of this species has been established, but some challenges associated with <em>in vitro</em> rooting remained to be improved. In this study, the <em>in vitro</em> rooting of <em>E. excelsum</em> plantlets were optimized by dark exposure, and the network of gene expression and endogenous hormones levels during dark-induced adventitious root (AR) formation were revealed. AR formation of <em>E. excelsum</em> plantlets were significantly promoted by dark exposure, especially by dark exposure for 15 d. In the stems of <em>E. excelsum</em> plantlets under the treatment of dark exposure for 15 d, lower level of abscisic acid (ABA), gibberellic acid 1 (GA<sub>1</sub>), isopentenyladenine (IP), isopentenyladenosine (IPA) and zeatin (ZT), as well as higher level of GA<sub>7</sub>, jasmonic acid (JA) and salicylic acid (SA), promoted the whole course of AR formation. The higher level of trans-zeatin riboside (TZR) and T-zeatin (TZT) promoted the elongation of dark-induced AR, while higher level of indole-3-acetic acid (IAA) stimulated the process of AR primordia formation. Differentially expressed genes (DEGs) involved in hormone biosynthesis, plant hormone signal transduction and phenylpropanoid biosynthesis participated in the regulation of dark-induced AR development. The weighted gene co-expression network (WGCNA) analysis identified five modules that had highly correlation with phytohormone contents, and numerous hub genes associated with carotenoid biosynthesis, tryptophan metabolism, zeatin biosynthesis, alpha-Linolenic acid metabolism, phenylalanine metabolism, plant hormone signal transduction and phenylpropanoid biosynthesis were revealed. Those result will provide technical reference for <em>in vitro</em> rooting of woody species, and promote biological conservation and genetic engineering of rare and endangered species.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000355/pdfft?md5=fc82457e7d910241bb0af92a757aa6f2&pid=1-s2.0-S2214662824000355-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Integrated transcriptome and hormonal analysis of darkness-induced adventitious rooting of Euryodendron excelsum H. T. 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In the stems of <em>E. excelsum</em> plantlets under the treatment of dark exposure for 15 d, lower level of abscisic acid (ABA), gibberellic acid 1 (GA<sub>1</sub>), isopentenyladenine (IP), isopentenyladenosine (IPA) and zeatin (ZT), as well as higher level of GA<sub>7</sub>, jasmonic acid (JA) and salicylic acid (SA), promoted the whole course of AR formation. The higher level of trans-zeatin riboside (TZR) and T-zeatin (TZT) promoted the elongation of dark-induced AR, while higher level of indole-3-acetic acid (IAA) stimulated the process of AR primordia formation. Differentially expressed genes (DEGs) involved in hormone biosynthesis, plant hormone signal transduction and phenylpropanoid biosynthesis participated in the regulation of dark-induced AR development. The weighted gene co-expression network (WGCNA) analysis identified five modules that had highly correlation with phytohormone contents, and numerous hub genes associated with carotenoid biosynthesis, tryptophan metabolism, zeatin biosynthesis, alpha-Linolenic acid metabolism, phenylalanine metabolism, plant hormone signal transduction and phenylpropanoid biosynthesis were revealed. 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引用次数: 0
摘要
Euryodendron excelsum H. T. Chang 是中国特有的珍稀濒危常绿树种。该物种的微繁殖系统已经建立,但与离体生根相关的一些难题仍有待改进。本研究通过黑暗暴露优化了E. excelsum小植株的离体生根,并揭示了黑暗诱导不定根(AR)形成过程中的基因表达网络和内源激素水平。黑暗暴露能显著促进 E. excelsum 小植株的不定根形成,尤其是黑暗暴露 15 d。在黑暗暴露15 d的Extelsum小株茎中,较低水平的脱落酸(ABA)、赤霉素1(GA1)、异戊烯基腺嘌呤(IP)、异戊烯基腺苷(IPA)和玉米素(ZT),以及较高水平的GA7、茉莉酸(JA)和水杨酸(SA)促进了AR形成的整个过程。较高水平的反玉米素核苷(TZR)和T-玉米素(TZT)促进了暗诱导AR的伸长,而较高水平的吲哚-3-乙酸(IAA)刺激了AR初生茎的形成过程。参与激素生物合成、植物激素信号转导和苯丙酮生物合成的差异表达基因参与了暗诱导AR发育的调控。加权基因共表达网络(WGCNA)分析发现了与植物激素含量高度相关的五个模块,并揭示了与类胡萝卜素生物合成、色氨酸代谢、玉米素生物合成、α-亚麻酸代谢、苯丙氨酸代谢、植物激素信号转导和苯丙类生物合成相关的众多中枢基因。这些成果将为木本植物离体生根提供技术参考,促进珍稀濒危物种的生物保护和基因工程研究。
Integrated transcriptome and hormonal analysis of darkness-induced adventitious rooting of Euryodendron excelsum H. T. Chang during in vitro propagation
Euryodendron excelsum H. T. Chang, a rare and endangered evergreen tree that is endemic to China. The micropropagation system of this species has been established, but some challenges associated with in vitro rooting remained to be improved. In this study, the in vitro rooting of E. excelsum plantlets were optimized by dark exposure, and the network of gene expression and endogenous hormones levels during dark-induced adventitious root (AR) formation were revealed. AR formation of E. excelsum plantlets were significantly promoted by dark exposure, especially by dark exposure for 15 d. In the stems of E. excelsum plantlets under the treatment of dark exposure for 15 d, lower level of abscisic acid (ABA), gibberellic acid 1 (GA1), isopentenyladenine (IP), isopentenyladenosine (IPA) and zeatin (ZT), as well as higher level of GA7, jasmonic acid (JA) and salicylic acid (SA), promoted the whole course of AR formation. The higher level of trans-zeatin riboside (TZR) and T-zeatin (TZT) promoted the elongation of dark-induced AR, while higher level of indole-3-acetic acid (IAA) stimulated the process of AR primordia formation. Differentially expressed genes (DEGs) involved in hormone biosynthesis, plant hormone signal transduction and phenylpropanoid biosynthesis participated in the regulation of dark-induced AR development. The weighted gene co-expression network (WGCNA) analysis identified five modules that had highly correlation with phytohormone contents, and numerous hub genes associated with carotenoid biosynthesis, tryptophan metabolism, zeatin biosynthesis, alpha-Linolenic acid metabolism, phenylalanine metabolism, plant hormone signal transduction and phenylpropanoid biosynthesis were revealed. Those result will provide technical reference for in vitro rooting of woody species, and promote biological conservation and genetic engineering of rare and endangered species.
期刊介绍:
Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.