早产母山羊子宫输卵管的组织形态学和组织化学研究

Rusul Hazim, Masarat S. Al-Mayahi
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摘要

本研究旨在考察早产雌性本地山羊子宫输卵管的组织形态学和组织化学特征。此次调查共使用了 10 个子宫颈样本。这些样本来自屠宰场,年龄在 3 至 5 个月之间。组织学技术包括采用标准方法制备组织切片。这需要应用一般和特殊的组织化学染色法。采集的子宫管样本用 10%的中性缓冲福尔马林溶液保存。 采集后,我们立即对子宫管的大体形态、重量和长度进行了测量。采用标准组织学程序的方法。使用一般和特殊组织化学染色法制备组织化学切片。大体检查显示,双侧子宫输卵管由粗短的盘状前韧带、细长的安瓿和峡部组成,峡部是连接子宫的最小且最直的部分。在显微镜下,根据组织学特征的变化,确定了三个不同的区域。数据显示,幼猫的子宫管尚未完全发育成熟,而未成熟母猫的组织学和组织化学特征与幼猫相比发生了显著变化。另一方面,成熟雌猫表明断奶后阶段在动物发育中起着关键作用。组织化学方法能具体识别变化,在峡部区域观察到特别明显的阳性反应,与 PAS、AB 和 PAS-AB 组合明显反应。总之,最新数据显示,与其他哺乳动物相比,特别是与啮齿类动物相比,本地兔子的子宫管有明显的变化,子宫管的厚度在统计上有显著增加(P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histomorphological and Histochemical study of the uterine tubes in the premature female goats
This study aimed to examine the histomorphological and histochemical characteristics of the uterine tubes in premature female local goats. For this particular investigation, a total of 10 samples of uterine tubes were used. These samples were obtained from slaughterhouses and were taken from individuals aged between 3 and 5 months. The histological technique involved the execution of a standard approach to prepare histological sections. This was achieved by applying both general and particular Histochemical stains. Uterine tube samples were obtained and stored using a solution of 10% neutral buffered formalin for preservation.                                                                                               Immediately after collection, we conducted measurements of the uterine tube's gross morphology, weight, and length. By employing the methodology of standard histology procedure. Histochemical sections were prepared using both general and specific histochemical stains. The gross examination showed the presence of bilateral uterine tubes consisting of a thick, short, coiled preampulla, a thin, long ampulla, and the isthmus, which is the smallest and straightest section connecting to the uterus. Under microscopic examination, three distinct regions were identified based on variations in their histological characteristics. The data showed that the uterine tubes in kittens were not fully grown, while immature does exhibited significant histological and histochemical changes compared to kittens. On the other hand, mature does indicated that the post-weaning phase plays a crucial role in the development of the animals. The histochemical method specifically identified changes, with a particularly significant positive reaction observed in the isthmus region, which clearly reacted with PAS, AB, and a combination of PAS-AB. To summarise, the latest data revealed distinct variations in the uterine tubes of local rabbits compared to other mammals, particularly those belonging to the rodentia species.The uterine tube exhibited a statistically significant increase in thickness (P<0.05).The uterine tube was subjected to histochemical analysis utilising Haematoxylin and eosin, Alcian blue, Masson trichrome, Periodic acid, and Schiff reagent (PAS) stains..
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