{"title":"[利用 3.0 T MRI 的 GluCEST 成像评估肾缺血再灌注损伤时肾脏氧化还原代谢的实验研究]。","authors":"W W Mi, Z Y Xing, L Pan, X T Yu, J Chen, W Xing","doi":"10.3760/cma.j.cn112138-20240123-00064","DOIUrl":null,"url":null,"abstract":"<p><p><b>Objective:</b> To investigate the feasibility of 3.0 T glutamate chemical exchange saturation transfer (GluCEST) imaging in evaluating renal redox metabolism in renal ischemia-reperfusion injury (IRI). <b>Methods:</b> Rabbits in the IRI group (<i>n</i>=56) underwent surgery by clamping the left renal artery for 45 min and then releasing to establish IRI. Rabbits in the sham group (<i>n</i>=8) underwent the same operation without clamping the left renal artery. GluCEST MRI was performed before and at 1 h, 12 h, 1 day, 3 days, 7 days, and 14 days after the operations, with eight rabbits in the IRI group sacrificed immediately after each scanning and eight in the sham group sacrificed at 14 days after scanning. The left kidneys were removed for histopathological examination and reactive oxygen species (ROS) fluorescence staining. Differences in the magnetic resonance ratio asymmetry (MTR<sub>asym</sub>) of the renal cortex and outer medulla among different groups were compared. Correlations between the MTR<sub>asym</sub> and ROS were analyzed. <b>Results:</b> The MTR<sub>asym</sub> of the renal cortex in the sham and IRI subgroups were higher than that of the outer medulla (<i>t</i>=8.16, <i>P</i><0.001; <i>t</i>=4.78, <i>P</i>=0.002; <i>t</i>=4.94, <i>P</i>=0.002; <i>t</i>=5.76, <i>P</i>=0.001, <i>t</i>=6.68, <i>P</i><0.001; <i>t</i>=6.40, <i>P</i><0.001; <i>t</i>=5.16, <i>P</i>=0.001; <i>t</i>=3.30, <i>P</i>=0.013). The MTR<sub>asym</sub> of the renal cortex and outer medulla in the IRI-1h, IRI-12h, IRI-1d, IRI-3d, IRI-7d, and IRI-14d groups were lower than in the sham and IRI-pre groups (all <i>P</i><0.05). The MTR<sub>asym</sub> of the renal cortex and outer medulla in the IRI-1h group were lower than in the IRI-12h, IRI-1d, IRI-3d, IRI-7d, and IRI-14d groups (all <i>P</i><0.05). The MTR<sub>asym</sub> of the renal cortex in the IRI-12h group was lower than in the IRI-7d and IRI-14d groups (1.84%±0.09% vs.2.42%±0.19%, 2.41%±0.31%, all <i>P</i><0.05). The MTR<sub>asym</sub> of the renal cortex in the IRI-1d group was lower than in the IRI-7d group (1.99%±0.17% vs. 2.42%±0.19%, <i>P</i>=0.008). The MTR<sub>asym</sub> of the outer medulla in the IRI-12h group was lower than in the IRI-3d, IRI-7d, and IRI-14d groups (1.32%±0.27% vs. 1.79%±0.31%, 1.98%±0.18%, 1.66%±0.40%, respectively, all <i>P</i><0.05]. The MTR<sub>asym</sub> of the outer medulla in the IRI-7d group was higher than in the IRI-1d and IRI-14d groups (1.98%±0.18% vs. 1.52%±0.31%, 1.66%±0.40%, all <i>P</i><0.05). The MTR<sub>asym</sub> of the renal cortex and outer medulla had a strong negative correlation with the mean fluorescence intensity of ROS (<i>ρ</i>=-0.889, <i>P</i><0.001; <i>ρ</i>=-0.784, <i>P</i><0.001). <b>Conclusion:</b> 3.0 T GluCEST imaging can indirectly reflect the changes of renal redox metabolism in renal IRI.</p>","PeriodicalId":68309,"journal":{"name":"中华内科杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Experimental study on evaluating renal redox metabolism in renal ischemia-reperfusion injury using GluCEST imaging with 3.0 T MRI].\",\"authors\":\"W W Mi, Z Y Xing, L Pan, X T Yu, J Chen, W Xing\",\"doi\":\"10.3760/cma.j.cn112138-20240123-00064\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Objective:</b> To investigate the feasibility of 3.0 T glutamate chemical exchange saturation transfer (GluCEST) imaging in evaluating renal redox metabolism in renal ischemia-reperfusion injury (IRI). <b>Methods:</b> Rabbits in the IRI group (<i>n</i>=56) underwent surgery by clamping the left renal artery for 45 min and then releasing to establish IRI. Rabbits in the sham group (<i>n</i>=8) underwent the same operation without clamping the left renal artery. GluCEST MRI was performed before and at 1 h, 12 h, 1 day, 3 days, 7 days, and 14 days after the operations, with eight rabbits in the IRI group sacrificed immediately after each scanning and eight in the sham group sacrificed at 14 days after scanning. The left kidneys were removed for histopathological examination and reactive oxygen species (ROS) fluorescence staining. Differences in the magnetic resonance ratio asymmetry (MTR<sub>asym</sub>) of the renal cortex and outer medulla among different groups were compared. Correlations between the MTR<sub>asym</sub> and ROS were analyzed. <b>Results:</b> The MTR<sub>asym</sub> of the renal cortex in the sham and IRI subgroups were higher than that of the outer medulla (<i>t</i>=8.16, <i>P</i><0.001; <i>t</i>=4.78, <i>P</i>=0.002; <i>t</i>=4.94, <i>P</i>=0.002; <i>t</i>=5.76, <i>P</i>=0.001, <i>t</i>=6.68, <i>P</i><0.001; <i>t</i>=6.40, <i>P</i><0.001; <i>t</i>=5.16, <i>P</i>=0.001; <i>t</i>=3.30, <i>P</i>=0.013). The MTR<sub>asym</sub> of the renal cortex and outer medulla in the IRI-1h, IRI-12h, IRI-1d, IRI-3d, IRI-7d, and IRI-14d groups were lower than in the sham and IRI-pre groups (all <i>P</i><0.05). The MTR<sub>asym</sub> of the renal cortex and outer medulla in the IRI-1h group were lower than in the IRI-12h, IRI-1d, IRI-3d, IRI-7d, and IRI-14d groups (all <i>P</i><0.05). The MTR<sub>asym</sub> of the renal cortex in the IRI-12h group was lower than in the IRI-7d and IRI-14d groups (1.84%±0.09% vs.2.42%±0.19%, 2.41%±0.31%, all <i>P</i><0.05). The MTR<sub>asym</sub> of the renal cortex in the IRI-1d group was lower than in the IRI-7d group (1.99%±0.17% vs. 2.42%±0.19%, <i>P</i>=0.008). The MTR<sub>asym</sub> of the outer medulla in the IRI-12h group was lower than in the IRI-3d, IRI-7d, and IRI-14d groups (1.32%±0.27% vs. 1.79%±0.31%, 1.98%±0.18%, 1.66%±0.40%, respectively, all <i>P</i><0.05]. The MTR<sub>asym</sub> of the outer medulla in the IRI-7d group was higher than in the IRI-1d and IRI-14d groups (1.98%±0.18% vs. 1.52%±0.31%, 1.66%±0.40%, all <i>P</i><0.05). The MTR<sub>asym</sub> of the renal cortex and outer medulla had a strong negative correlation with the mean fluorescence intensity of ROS (<i>ρ</i>=-0.889, <i>P</i><0.001; <i>ρ</i>=-0.784, <i>P</i><0.001). <b>Conclusion:</b> 3.0 T GluCEST imaging can indirectly reflect the changes of renal redox metabolism in renal IRI.</p>\",\"PeriodicalId\":68309,\"journal\":{\"name\":\"中华内科杂志\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华内科杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/cma.j.cn112138-20240123-00064\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华内科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/cma.j.cn112138-20240123-00064","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的研究 3.0 T 谷氨酸化学交换饱和转移（GluCEST）成像在评估肾缺血再灌注损伤（IRI）中肾脏氧化还原代谢的可行性。方法：IRI组（56只）的兔子接受手术，夹闭左肾动脉45分钟，然后松开以建立IRI。假手术组（8 只）的兔子接受同样的手术，但不夹闭左肾动脉。在手术前和手术后 1 小时、12 小时、1 天、3 天、7 天和 14 天分别进行 GluCEST MRI 扫描，每次扫描后立即处死 IRI 组的 8 只兔子，处死假组的 8 只兔子则在扫描后 14 天处死。取出左肾进行组织病理学检查和活性氧（ROS）荧光染色。比较不同组间肾皮质和外髓质磁共振比值不对称性（MTRasym）的差异。分析了 MTRasym 与 ROS 之间的相关性。结果假性和IRI亚组肾皮质的MTRasym高于外髓质（t=8.16，Pt=4.78，P=0.002；t=4.94，P=0.002；t=5.76，P=0.001；t=6.68，Pt=6.40，Pt=5.16，P=0.001；t=3.30，P=0.013）。IRI-1h、IRI-12h、IRI-1d、IRI-3d、IRI-7d 和 IRI-14d 组肾皮质和外髓的 MTRasym 均低于假组和 IRI 前组（IRI-1h 组肾皮质和外髓的所有 Pasym 均低于 IRI-12h、IRI-1d、IRI-3d 和 IRI-14d 组）、IRI-1d、IRI-3d、IRI-7d 和 IRI-14d 组（IRI-12h 组肾脏皮质的所有 Pasym 均低于 IRI-7d 和 IRI-14d 组（1.84%±0.09% vs. 2IRI-1d组肾脏皮质的所有Pasym均低于IRI-7d组（1.99%±0.17% vs. 2.42%±0.19%，P=0.008）。IRI-12h组外侧髓质的MTRasym低于IRI-3d、IRI-7d和IRI-14d组（分别为1.32%±0.27% vs. 1.79%±0.31%、1.98%±0.18%、1.66%±0.40%，IRI-7d组外侧髓质的所有Pasym高于IRI-1d和IRI-14d组（1.98%±0.18% vs. 1.52%±0.31%, 1.66%±0.40%, IRI-7d组肾皮质和外髓的所有Pasym与ROS的平均荧光强度呈强负相关（ρ=-0.889，Pρ=-0.784，PC结论：3.0 T GluCEST成像可间接反映肾脏IRI时肾脏氧化还原代谢的变化。
[Experimental study on evaluating renal redox metabolism in renal ischemia-reperfusion injury using GluCEST imaging with 3.0 T MRI].
Objective: To investigate the feasibility of 3.0 T glutamate chemical exchange saturation transfer (GluCEST) imaging in evaluating renal redox metabolism in renal ischemia-reperfusion injury (IRI). Methods: Rabbits in the IRI group (n=56) underwent surgery by clamping the left renal artery for 45 min and then releasing to establish IRI. Rabbits in the sham group (n=8) underwent the same operation without clamping the left renal artery. GluCEST MRI was performed before and at 1 h, 12 h, 1 day, 3 days, 7 days, and 14 days after the operations, with eight rabbits in the IRI group sacrificed immediately after each scanning and eight in the sham group sacrificed at 14 days after scanning. The left kidneys were removed for histopathological examination and reactive oxygen species (ROS) fluorescence staining. Differences in the magnetic resonance ratio asymmetry (MTRasym) of the renal cortex and outer medulla among different groups were compared. Correlations between the MTRasym and ROS were analyzed. Results: The MTRasym of the renal cortex in the sham and IRI subgroups were higher than that of the outer medulla (t=8.16, P<0.001; t=4.78, P=0.002; t=4.94, P=0.002; t=5.76, P=0.001, t=6.68, P<0.001; t=6.40, P<0.001; t=5.16, P=0.001; t=3.30, P=0.013). The MTRasym of the renal cortex and outer medulla in the IRI-1h, IRI-12h, IRI-1d, IRI-3d, IRI-7d, and IRI-14d groups were lower than in the sham and IRI-pre groups (all P<0.05). The MTRasym of the renal cortex and outer medulla in the IRI-1h group were lower than in the IRI-12h, IRI-1d, IRI-3d, IRI-7d, and IRI-14d groups (all P<0.05). The MTRasym of the renal cortex in the IRI-12h group was lower than in the IRI-7d and IRI-14d groups (1.84%±0.09% vs.2.42%±0.19%, 2.41%±0.31%, all P<0.05). The MTRasym of the renal cortex in the IRI-1d group was lower than in the IRI-7d group (1.99%±0.17% vs. 2.42%±0.19%, P=0.008). The MTRasym of the outer medulla in the IRI-12h group was lower than in the IRI-3d, IRI-7d, and IRI-14d groups (1.32%±0.27% vs. 1.79%±0.31%, 1.98%±0.18%, 1.66%±0.40%, respectively, all P<0.05]. The MTRasym of the outer medulla in the IRI-7d group was higher than in the IRI-1d and IRI-14d groups (1.98%±0.18% vs. 1.52%±0.31%, 1.66%±0.40%, all P<0.05). The MTRasym of the renal cortex and outer medulla had a strong negative correlation with the mean fluorescence intensity of ROS (ρ=-0.889, P<0.001; ρ=-0.784, P<0.001). Conclusion: 3.0 T GluCEST imaging can indirectly reflect the changes of renal redox metabolism in renal IRI.
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