UBE2C 作为乳腺癌的免疫相关生物标记物：基于多个数据库的研究。

Q2 Medicine

Chinese Medical Sciences Journal Pub Date : 2024-09-01 DOI:10.24920/004340

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引用次数: 0

摘要

目的利用多个数据库筛选乳腺癌（BRCA）的靶基因 UBE2C 并探讨其预后价值和免疫相关性。方法从基因表达综合数据库（Gene Expresssion Omnibus database，GEO）下载 BRCA 的微阵列表达数据集，并对其进行分析，以获得差异表达基因（DEGs）。通过构建和可视化 DEGs 的蛋白质-蛋白质相互作用网络，获得枢纽基因。然后利用 R 语言、STRING 和 Cytoscape 确定了关键基因 UBE2C，并利用外部数据集、癌症基因组图谱（TCGA）和定量实时 PCR（qRT-PCR）验证了 UBE2C 的差异表达。使用 R 语言、TIMER 和基因组富集分析（Gene Set Enrichment Analysis，GSEA）探讨了 UBE2C 在 BRCA 中的预后价值和免疫学相关性。预后分析表明，UBE2C是一个独立的预后因素。UBE2C 的高表达与 BRCA 组织中 B 细胞、CD4+ T 细胞、CD8+ T 细胞、巨噬细胞和髓树突状细胞的免疫浸润水平下降有关。UBE2C 在 BRCA 中的表达与 PDCD1、CD274 和 CTLA4 的表达有显著相关性。UBE2C 的表达与肿瘤突变负荷和微卫星不稳定性呈正相关。结论 BRCA组织中UBE2C的表达可预测BRCA患者的存活率和预后。结论 UBE2C 在 BRCA 组织中的表达可预测 BRCA 患者的存活率和预后，而且与 BRCA 免疫微环境密切相关，可预测 BRCA 患者免疫治疗的效果。因此，UBE2C可能是一种潜在的与免疫相关的BRCA预后生物标志物。

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UBE2C as an Immune-Related Biomarker for Breast Cancer: A Study Based on Multiple Databases

Objective

To screen the target genes that are associated with survival of breast cancer (BRCA) and explore their prognostic values and immune correlations with BRCA using multiple databases..

Methods

The microarray expression datasets of BRCA were downloaded from the Gene Expresssion Omnibus database (GEO) and analyzed to obtain differentially expressed genes (DEGs). Hub genes were obtained by constructing and visualizing the protein-protein interaction network of DEGs. The key gene was determined using R language, STRING, and Cytoscape, and the differential expression of the key gene was verified using external datasets The Cancer Genome Atlas (TCGA) and quantitative real-time PCR (qRT-PCR) for BRCA tissues of 37 patients. The prognostic value and immunological correlation of UBE2C in BRCA were explored using R language, TIMER, and Gene Set Enrichment Analysis (GSEA).

Results

Of 10 hub genes seleceed from 302 DEGS, UBE2C was identified as the gene associated with BRCA survival. The expression of UBE2C was differentially upregulated in BRCA, as verified by TCGA and qRT-PCR. Prognostic analysis revealed that UBE2C served as an independent prognostic factor. High expression of UBE2C was associated with decreased immune infiltration levels of B cells, CD4+ T cells, CD8+ T cells, macrophages, and myeloid dendritic cells in BRCA tissue. The expression of UBE2C in BRCA showed a significant correlation with immune checkpoints genes PDCD1, CD274, and CTLA4 expressions. There was a positive correlation between the expression of UBE2C and the tumor mutational burden and microsatellite instability. GSEA demonstrated that UBE2C expression significantly enriched 786 immune-related gene sets.

Conclusions

UBE2C expression in BRCA tissues is closely related to the BRCA immune microenvironment and showes predictive values on the survivals and prognosis of BRCA patients and the effecacy of immunotherapy. UBE2C may be an potential immune-related prognostic biomarker for BRCA.

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来源期刊

Chinese Medical Sciences Journal Medicine-Medicine (all)

CiteScore

2.40

自引率

0.00%

发文量

1275