比较基因组分析揭示猴痘病毒进化背后的驱动力，并阐明 ATC 三核苷酸基序的作用

IF 5.5 2区医学 Q1 VIROLOGY

Virus Evolution Pub Date : 2024-05-31 DOI:10.1093/ve/veae043

Preeti Agarwal, Nityendra Shukla, Ajay Bhatia, Sahil Mahfooz, Jitendra Narayan

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引用次数: 0

摘要

猴痘（MPOX）是一种人畜共患病，1970 年起源于非洲西部和中部，最近在 100 多个国家暴发流行。对 404 个猴痘病毒（MPXV）基因组的比较分析表明，微卫星的丰度和密度发生了显著变化，尤其是在支系 I、支系 IIa 和支系 IIb 中。每个支系都显示出独特的微卫星图案，其中有 26 个保守位点是 MPXV 特有的，这表明它们有可能成为诊断中的分子标记。此外，MPXV 基因组中的九个基因具有十个与表面蛋白合成和宿主控制有关的可变热点微卫星区。值得注意的是，基因 OPG153，尤其是 SSR 位点"(ATC)n"，随着时间的推移在不同品系之间的变异最为明显，并在病毒在宿主细胞内的致病过程中发挥作用。这些发现不仅加深了我们对 MPXV 独特分子特征的了解，还为潜在的致病和进化意义提供了宝贵的见解。

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Comparative genome analysis reveals driving forces behind Monkeypox virus evolution and sheds light on the role of ATC trinucleotide motif

Monkeypox (MPOX), a zoonotic disease originating in Western and Central Africa in 1970, has seen a recent surge in outbreaks across 100+ countries. A comparative analysis of 404 Monkeypox virus (MPXV) genomes revealed notable changes in microsatellite abundance and density, especially within Clades I, IIa, and IIb. Each clade exhibited unique microsatellite motifs, with twenty-six conserved loci specific to MPXV, suggesting their potential as molecular markers in diagnostics. Additionally, nine genes in the MPXV genome featured ten variable hotspot microsatellite regions associated with surface protein synthesis and host control. Notably, gene OPG153, especially at the SSR locus ‘(ATC)n’, exhibited the most pronounced variations among lineages over time and plays a role in virus pathogenesis within the host cell. These findings not only enhance our understanding of MPXV unique molecular profile but also offer valuable insights into potential pathogenic and evolutionary implications.

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来源期刊

Virus Evolution Immunology and Microbiology-Microbiology

CiteScore

10.50

自引率

5.70%

发文量

108

审稿时长

14 weeks

期刊介绍： Virus Evolution is a new Open Access journal focusing on the long-term evolution of viruses, viruses as a model system for studying evolutionary processes, viral molecular epidemiology and environmental virology. The aim of the journal is to provide a forum for original research papers, reviews, commentaries and a venue for in-depth discussion on the topics relevant to virus evolution.