Bruna Alícia R. Paiva, Anne Preveaux, Armelle Darrasse, Adriane Wendland, Maurício Rossato, Abi S. A. Marques, Marisa A. S. V. Ferreira, Marie-Agnès Jacques
{"title":"对巴西中部普通细菌性枯萎病的调查发现了感染普通豆类的第三种黄单胞菌","authors":"Bruna Alícia R. Paiva, Anne Preveaux, Armelle Darrasse, Adriane Wendland, Maurício Rossato, Abi S. A. Marques, Marisa A. S. V. Ferreira, Marie-Agnès Jacques","doi":"10.1007/s40858-024-00655-3","DOIUrl":null,"url":null,"abstract":"<p>A survey of common bacterial blight (CBB) was conducted in common bean fields in Brazil, in the state of Goiás and in the Federal District. Isolation from symptomatic leaves on a semi-selective medium yielded yellow, mucoid colonies typical of <i>Xanthomonas</i>, and 161 pure cultures were obtained. PCR with specific primers for <i>X. phaseoli</i> pv. <i>phaseoli</i> and <i>X. citri</i> pv. <i>fuscans</i> was performed and confirmed the presence of both fuscans and GL2 strains of <i>X. citri</i> pv. <i>fuscans</i> and the presence of <i>X. phaseoli</i> pv. <i>phaseoli.</i> However, for 81 isolates, PCR results were negative. Housekeeping genes <i>gyrB</i> and <i>rpoD</i> sequences placed these strains in the provisionally named <i>X. cannabis</i> species clade, clustering pv. <i>zinniae</i>, pv. <i>esculenti</i>, and the strain Nyagatare, isolated from beans in Rwanda in 2013. The identification of a subset of 20 strains was complemented by a positive PCR with <i>Xanthomonas</i>-specific primers that amplify a portion of the <i>gumD</i> gene and the induction of hypersensitive reaction in tomato leaves within 24-48 hours. Pathogenicity was confirmed by inoculation on the French common bean cv. Flavert and the Brazilian common bean cv. BRS Ártico. Symptoms such as white spots along the leaf blade, leaf curling, and wilting developed in inoculated plants. Partial <i>gyrB</i> and <i>rpoD</i> sequences analyses revealed identity values ranging from 98.3 to 100% between the Brazilian isolates and strain Nyagatare, proposed as <i>X. cannabis</i> pv. <i>phaseoli</i>.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":"28 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A survey of common bacterial blight in Central Brazil reveals a third Xanthomonas species infecting common bean\",\"authors\":\"Bruna Alícia R. Paiva, Anne Preveaux, Armelle Darrasse, Adriane Wendland, Maurício Rossato, Abi S. A. Marques, Marisa A. S. V. Ferreira, Marie-Agnès Jacques\",\"doi\":\"10.1007/s40858-024-00655-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>A survey of common bacterial blight (CBB) was conducted in common bean fields in Brazil, in the state of Goiás and in the Federal District. Isolation from symptomatic leaves on a semi-selective medium yielded yellow, mucoid colonies typical of <i>Xanthomonas</i>, and 161 pure cultures were obtained. PCR with specific primers for <i>X. phaseoli</i> pv. <i>phaseoli</i> and <i>X. citri</i> pv. <i>fuscans</i> was performed and confirmed the presence of both fuscans and GL2 strains of <i>X. citri</i> pv. <i>fuscans</i> and the presence of <i>X. phaseoli</i> pv. <i>phaseoli.</i> However, for 81 isolates, PCR results were negative. Housekeeping genes <i>gyrB</i> and <i>rpoD</i> sequences placed these strains in the provisionally named <i>X. cannabis</i> species clade, clustering pv. <i>zinniae</i>, pv. <i>esculenti</i>, and the strain Nyagatare, isolated from beans in Rwanda in 2013. The identification of a subset of 20 strains was complemented by a positive PCR with <i>Xanthomonas</i>-specific primers that amplify a portion of the <i>gumD</i> gene and the induction of hypersensitive reaction in tomato leaves within 24-48 hours. Pathogenicity was confirmed by inoculation on the French common bean cv. Flavert and the Brazilian common bean cv. BRS Ártico. Symptoms such as white spots along the leaf blade, leaf curling, and wilting developed in inoculated plants. Partial <i>gyrB</i> and <i>rpoD</i> sequences analyses revealed identity values ranging from 98.3 to 100% between the Brazilian isolates and strain Nyagatare, proposed as <i>X. cannabis</i> pv. <i>phaseoli</i>.</p>\",\"PeriodicalId\":23354,\"journal\":{\"name\":\"Tropical Plant Pathology\",\"volume\":\"28 1\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s40858-024-00655-3\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00655-3","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
A survey of common bacterial blight in Central Brazil reveals a third Xanthomonas species infecting common bean
A survey of common bacterial blight (CBB) was conducted in common bean fields in Brazil, in the state of Goiás and in the Federal District. Isolation from symptomatic leaves on a semi-selective medium yielded yellow, mucoid colonies typical of Xanthomonas, and 161 pure cultures were obtained. PCR with specific primers for X. phaseoli pv. phaseoli and X. citri pv. fuscans was performed and confirmed the presence of both fuscans and GL2 strains of X. citri pv. fuscans and the presence of X. phaseoli pv. phaseoli. However, for 81 isolates, PCR results were negative. Housekeeping genes gyrB and rpoD sequences placed these strains in the provisionally named X. cannabis species clade, clustering pv. zinniae, pv. esculenti, and the strain Nyagatare, isolated from beans in Rwanda in 2013. The identification of a subset of 20 strains was complemented by a positive PCR with Xanthomonas-specific primers that amplify a portion of the gumD gene and the induction of hypersensitive reaction in tomato leaves within 24-48 hours. Pathogenicity was confirmed by inoculation on the French common bean cv. Flavert and the Brazilian common bean cv. BRS Ártico. Symptoms such as white spots along the leaf blade, leaf curling, and wilting developed in inoculated plants. Partial gyrB and rpoD sequences analyses revealed identity values ranging from 98.3 to 100% between the Brazilian isolates and strain Nyagatare, proposed as X. cannabis pv. phaseoli.
期刊介绍:
Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.
Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection.
The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines.
Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.