对巴西中部普通细菌性枯萎病的调查发现了感染普通豆类的第三种黄单胞菌

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences
Bruna Alícia R. Paiva, Anne Preveaux, Armelle Darrasse, Adriane Wendland, Maurício Rossato, Abi S. A. Marques, Marisa A. S. V. Ferreira, Marie-Agnès Jacques
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引用次数: 0

摘要

对巴西戈亚斯州和联邦区的普通豆田进行了普通细菌性枯萎病(CBB)调查。在半选择性培养基上从有症状的叶片中分离出了黄单胞菌典型的黄色粘液菌落,并获得了 161 个纯培养物。用 X. phaseoli pv. phaseoli 和 X. citri pv. fuscans 的特异引物进行 PCR 检测,结果证实存在 fuscans 和 GL2 株 X. citri pv. fuscans 以及 X. phaseoli pv. phaseoli。但有 81 个分离株的 PCR 结果为阴性。管家基因 gyrB 和 rpoD 序列将这些菌株归入暂时命名的大麻 X. 种支系,与 zinniae pv.、esculenti pv.以及 2013 年从卢旺达豆类中分离出的 Nyagatare 菌株聚类。利用黄单胞菌特异性引物(扩增了 gumD 基因的一部分)进行 PCR 检测,结果呈阳性,并在 24-48 小时内诱导番茄叶片产生超敏反应,从而确定了 20 株菌株的子集。通过接种法国普通豆品种 Flavert 和巴西普通豆品种,证实了致病性。Flavert和巴西蚕豆品种BRS Ártico上接种,证实了其致病性。BRS Ártico。接种植株出现叶片白斑、叶片卷曲和枯萎等症状。部分 gyrB 和 rpoD 序列分析表明,巴西分离物与菌株 Nyagatare 之间的同一性值从 98.3 到 100%不等,被认为是 X. cannabis pv. phaseoli。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A survey of common bacterial blight in Central Brazil reveals a third Xanthomonas species infecting common bean

A survey of common bacterial blight in Central Brazil reveals a third Xanthomonas species infecting common bean

A survey of common bacterial blight (CBB) was conducted in common bean fields in Brazil, in the state of Goiás and in the Federal District. Isolation from symptomatic leaves on a semi-selective medium yielded yellow, mucoid colonies typical of Xanthomonas, and 161 pure cultures were obtained. PCR with specific primers for X. phaseoli pv. phaseoli and X. citri pv. fuscans was performed and confirmed the presence of both fuscans and GL2 strains of X. citri pv. fuscans and the presence of X. phaseoli pv. phaseoli. However, for 81 isolates, PCR results were negative. Housekeeping genes gyrB and rpoD sequences placed these strains in the provisionally named X. cannabis species clade, clustering pv. zinniae, pv. esculenti, and the strain Nyagatare, isolated from beans in Rwanda in 2013. The identification of a subset of 20 strains was complemented by a positive PCR with Xanthomonas-specific primers that amplify a portion of the gumD gene and the induction of hypersensitive reaction in tomato leaves within 24-48 hours. Pathogenicity was confirmed by inoculation on the French common bean cv. Flavert and the Brazilian common bean cv. BRS Ártico. Symptoms such as white spots along the leaf blade, leaf curling, and wilting developed in inoculated plants. Partial gyrB and rpoD sequences analyses revealed identity values ranging from 98.3 to 100% between the Brazilian isolates and strain Nyagatare, proposed as X. cannabis pv. phaseoli.

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来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
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