稀有濒危的格兰瑟姆山茶花(Camellia granthamiana)的基因组组装。

GigaByte (Hong Kong, China) Pub Date : 2024-05-20 eCollection Date: 2024-01-01 DOI:10.46471/gigabyte.124
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引用次数: 0

摘要

葛氏山茶(Camellia granthamiana Sealy)是1955年在香港发现的稀有濒危茶树品种,为中国南方特有物种。尽管它具有很高的保护价值,但C. granthamiana的基因组资源却很有限。在此,我们结合 PacBio 长线程测序和 Omni-C 数据,提出了四倍体 C. granthamiana(2n = 4x = 60)的染色体级基因组草案。组装的基因组大小为 2.4 Gb,大部分序列锚定在 15 个假染色体上,类似于单倍体基因组。基因组的连续性很高,支架 N50 为 139.7 Mb,完整性也很高(97.8% BUSCO 得分)。我们的基因模型预测得出了 68,032 个蛋白质编码基因(BUSCO 得分为 90.9%)。我们注释了 1.65 Gb 的重复内容(占基因组的 68.48%)。我们的格兰山茶花基因组组装是研究格兰山茶花生物学、生态学以及与其他山茶花物种系统发育关系的宝贵资源,并为进一步的保护措施奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome assembly of the rare and endangered Grantham's camellia, Camellia granthamiana.

Grantham's camellia (Camellia granthamiana Sealy) is a rare and endangered tea species discovered in Hong Kong in 1955 and endemic to southern China. Despite its high conservation value, the genomic resources of C. granthamiana are limited. Here, we present a chromosome-scale draft genome of the tetraploid C. granthamiana (2n = 4x = 60), combining PacBio long-read sequencing and Omni-C data. The assembled genome size is ∼2.4 Gb, with most sequences anchored to 15 pseudochromosomes resembling a monoploid genome. The genome has high contiguity, with a scaffold N50 of 139.7 Mb, and high completeness (97.8% BUSCO score). Our gene model prediction resulted in 68,032 protein-coding genes (BUSCO score of 90.9%). We annotated 1.65 Gb of repeat content (68.48% of the genome). Our Grantham's camellia genome assembly is a valuable resource for investigating Grantham's camellia's biology, ecology, and phylogenomic relationships with other Camellia species, and provides a foundation for further conservation measures.

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CiteScore
2.60
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