Jiaxin Ren, Yaoyi Zhu, Yuying Nie, Mohan Zheng, Ainizati Hasimu, Ming Zhao, Yiming Zhao, Xiancan Ma, Zihang Yuan, Qi Li, Ayibaota Bahabayi, Zhonghui Zhang, Xingyue Zeng, Chen Liu
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Changes in GPR56+ cells in early LUAD patients were analysed, and the diagnostic significance of GPR56+ T cells for early LUAD was studied by receiver operating characteristic (ROC) curve analysis.</p><p><strong>Results: </strong>The expression of GPR56 in CD8+ T cells from early-stage LUAD patients was significantly greater than that in CD4+ T cells. The percentage of perforin-positive GPR56+ cells in early-stage LUAD patients was high. GPR56 levels in the T cells of LUAD patients were significantly lower than those in healthy controls. ROC analysis revealed that the area under the curve for the percentage of GPR56-positive CD8+ TEMRA cells to distinguish early-stage LUAD patients from healthy individuals- reached 0.7978.</p><p><strong>Conclusion: </strong>The decreased expression of GPR56 in the peripheral blood of early-stage LUAD patients correlated with perforin levels, reflecting compromised antitumor immunity and aiding early-stage LUAD screening.</p>","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"843-856"},"PeriodicalIF":2.9000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differential GPR56 Expression in T Cell Subpopulations for Early-Stage Lung Adenocarcinoma Patient Identification.\",\"authors\":\"Jiaxin Ren, Yaoyi Zhu, Yuying Nie, Mohan Zheng, Ainizati Hasimu, Ming Zhao, Yiming Zhao, Xiancan Ma, Zihang Yuan, Qi Li, Ayibaota Bahabayi, Zhonghui Zhang, Xingyue Zeng, Chen Liu\",\"doi\":\"10.1080/08820139.2024.2350549\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aimed to investigate the expression of GPR56 in the T cells of early-stage lung adenocarcinoma (LUAD) patients and clarify its diagnostic significance.</p><p><strong>Methods: </strong>Blood samples were collected from 32 patients with stage IA LUAD and 31 healthy controls. 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引用次数: 0
摘要
研究目的本研究旨在探讨GPR56在早期肺腺癌（LUAD）患者T细胞中的表达，并明确其诊断意义：方法：采集32名IA期LUAD患者和31名健康对照者的血液样本。流式细胞术分析了循环 T 细胞亚群中的 GPR56 和穿孔素。此外，还检测了穿孔素和 GPR56 表达之间的相关性。分析了早期 LUAD 患者 GPR56+ 细胞的变化，并通过接收器操作特征曲线（ROC）分析研究了 GPR56+ T 细胞对早期 LUAD 的诊断意义：结果：早期 LUAD 患者 CD8+ T 细胞中 GPR56 的表达明显高于 CD4+ T 细胞。早期 LUAD 患者中穿孔素阳性 GPR56+ 细胞的比例很高。LUAD患者T细胞中的GPR56水平明显低于健康对照组。ROC分析显示，GPR56阳性CD8+ TEMRA细胞百分比的曲线下面积达到0.7978，可用于区分早期LUAD患者和健康人：结论：GPR56在早期LUAD患者外周血中表达的减少与穿孔素水平相关，反映了抗肿瘤免疫力的下降，有助于早期LUAD的筛查。
Differential GPR56 Expression in T Cell Subpopulations for Early-Stage Lung Adenocarcinoma Patient Identification.
Objective: This study aimed to investigate the expression of GPR56 in the T cells of early-stage lung adenocarcinoma (LUAD) patients and clarify its diagnostic significance.
Methods: Blood samples were collected from 32 patients with stage IA LUAD and 31 healthy controls. GPR56 and perforin were analysed in circulating T-cell subsets by flow cytometry. In addition, a correlation between perforin and GPR56 expression was detected. Changes in GPR56+ cells in early LUAD patients were analysed, and the diagnostic significance of GPR56+ T cells for early LUAD was studied by receiver operating characteristic (ROC) curve analysis.
Results: The expression of GPR56 in CD8+ T cells from early-stage LUAD patients was significantly greater than that in CD4+ T cells. The percentage of perforin-positive GPR56+ cells in early-stage LUAD patients was high. GPR56 levels in the T cells of LUAD patients were significantly lower than those in healthy controls. ROC analysis revealed that the area under the curve for the percentage of GPR56-positive CD8+ TEMRA cells to distinguish early-stage LUAD patients from healthy individuals- reached 0.7978.
Conclusion: The decreased expression of GPR56 in the peripheral blood of early-stage LUAD patients correlated with perforin levels, reflecting compromised antitumor immunity and aiding early-stage LUAD screening.
期刊介绍：
Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.
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