Eisner Salamanca, Yi-Fan Wu, Lwin Moe Aung, Bor Rong Chiu, Mei Kuang Chen, Wei-Jen Chang, Ying Sui Sun
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In vivo experiments involved implanting zirconia dental implants into rabbit femurs and later evaluation through impact stability test, micro-CT, and histomorphometric measurements.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The results demonstrated that 25 and 50 W GDP allylamine grafting positively impacted MG-63 cell proliferation and increased alkaline phosphatase activity. Gene expression analysis revealed upregulation of OCN, OPG, and COL-I. Both 25 and 50 W GDP allylamine grafting significantly improved zirconia's surface properties (<i>p</i> < .05, <i>p</i> < .01, <i>p</i> < .001). 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引用次数: 0
摘要
目的：辉光放电等离子体（GDP）程序已被证明能有效地将烯丙基胺移植到氧化锆牙种植体表面，以增强骨结合。本研究探讨了使用不同能量设置（25、50、75、100 和 200 瓦）的辉光放电等离子体在体外和体内增强氧化锆牙种植体性能的情况：体外分析包括扫描电子显微镜、润湿性评估、能量色散 X 射线光谱等。体内实验包括将氧化锆牙科植入体植入兔子股骨，然后通过冲击稳定性测试、显微 CT 和组织形态测量进行评估：结果表明，25 W 和 50 W GDP 烯丙胺移植对 MG-63 细胞增殖和碱性磷酸酶活性的提高有积极影响。基因表达分析表明，OCN、OPG 和 COL-I 上调。25 W 和 50 W GDP 烯丙胺接枝都能显著改善氧化锆的表面性能（p 结论：烯丙胺接枝对氧化锆的表面性能有积极影响：本研究提出了一种增强氧化锆牙种植体表面生物活性的可行方法。
Allylamine coating on zirconia dental implant surface promotes osteogenic differentiation in vitro and accelerates osseointegration in vivo
Objectives
The glow discharge plasma (GDP) procedure has proven efficacy in grafting allylamine onto zirconia dental implant surfaces to enhance osseointegration. This study explored the enhancement of zirconia dental implant properties using GDP at different energy settings (25, 50, 75, 100, and 200 W) both in vitro and in vivo.
Materials and Methods
In vitro analyses included scanning electron microscopy, wettability assessment, energy-dispersive X-ray spectroscopy, and more. In vivo experiments involved implanting zirconia dental implants into rabbit femurs and later evaluation through impact stability test, micro-CT, and histomorphometric measurements.
Results
The results demonstrated that 25 and 50 W GDP allylamine grafting positively impacted MG-63 cell proliferation and increased alkaline phosphatase activity. Gene expression analysis revealed upregulation of OCN, OPG, and COL-I. Both 25 and 50 W GDP allylamine grafting significantly improved zirconia's surface properties (p < .05, p < .01, p < .001). However, only 25 W allylamine grafting with optimal energy settings promoted in vivo osseointegration and new bone formation while preventing bone level loss around the dental implant (p < .05, p < .01, p < .001).
Conclusions
This study presents a promising method for enhancing Zr dental implant surface's bioactivity.
期刊介绍：
Clinical Oral Implants Research conveys scientific progress in the field of implant dentistry and its related areas to clinicians, teachers and researchers concerned with the application of this information for the benefit of patients in need of oral implants. The journal addresses itself to clinicians, general practitioners, periodontists, oral and maxillofacial surgeons and prosthodontists, as well as to teachers, academicians and scholars involved in the education of professionals and in the scientific promotion of the field of implant dentistry.
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