{"title":"臭氧对甲氨蝶呤引起的小鼠睾丸损伤的保护作用。","authors":"Layasadat Khorsandi, Negar Varaa, Reza Dadfar, Sadegh Moradi Vastegani, Yousef Asadi-Fard, Akram Ahangarpour, Amirhesam Keshavarz-Zarjani","doi":"10.5935/1518-0557.20240041","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Methotrexate (MTX) is widely administered for the treatment of various cancers. However, MTX induces male reproductive toxicity. In the current study, the effect of ozone therapy (OT) on reducing the toxic effects of MTX in the mouse testicles has been investigated.</p><p><strong>Methods: </strong>Twenty-four mice were divided into four groups: control, OT (4 mg/kg ozone), MTX (20 mg/kg), and MTX + OT. Testosterone levels, histological changes, and oxidative stress biomarkers were assessed to evaluate the protective effects of OT.</p><p><strong>Results: </strong>The results demonstrated that MTX disrupted germinal epithelium, reduced serum testosterone levels, and enhanced oxidative stress in testicular tissue. However, treatment with OT attenuated these adverse effects. OT effectively restored the levels of antioxidant enzymes, such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD). OT reduced lipid peroxidation, as indicated by decreased malondialdehyde (MDA) levels. OT preserved normal spermatogenesis, improved morphometric parameters, and reduced histological changes by MTX. Moreover, OT effectively restored testosterone levels.</p><p><strong>Conclusions: </strong>OT protects against MTX-induced testicular damage by suppressing oxidative stress.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"464-470"},"PeriodicalIF":1.8000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11349260/pdf/","citationCount":"0","resultStr":"{\"title\":\"The protective effect of Ozone on the mice testicular damage induced by methotrexate.\",\"authors\":\"Layasadat Khorsandi, Negar Varaa, Reza Dadfar, Sadegh Moradi Vastegani, Yousef Asadi-Fard, Akram Ahangarpour, Amirhesam Keshavarz-Zarjani\",\"doi\":\"10.5935/1518-0557.20240041\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Methotrexate (MTX) is widely administered for the treatment of various cancers. However, MTX induces male reproductive toxicity. In the current study, the effect of ozone therapy (OT) on reducing the toxic effects of MTX in the mouse testicles has been investigated.</p><p><strong>Methods: </strong>Twenty-four mice were divided into four groups: control, OT (4 mg/kg ozone), MTX (20 mg/kg), and MTX + OT. Testosterone levels, histological changes, and oxidative stress biomarkers were assessed to evaluate the protective effects of OT.</p><p><strong>Results: </strong>The results demonstrated that MTX disrupted germinal epithelium, reduced serum testosterone levels, and enhanced oxidative stress in testicular tissue. However, treatment with OT attenuated these adverse effects. OT effectively restored the levels of antioxidant enzymes, such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD). OT reduced lipid peroxidation, as indicated by decreased malondialdehyde (MDA) levels. OT preserved normal spermatogenesis, improved morphometric parameters, and reduced histological changes by MTX. Moreover, OT effectively restored testosterone levels.</p><p><strong>Conclusions: </strong>OT protects against MTX-induced testicular damage by suppressing oxidative stress.</p>\",\"PeriodicalId\":46364,\"journal\":{\"name\":\"Jornal Brasileiro de Reproducao Assistida\",\"volume\":\" \",\"pages\":\"464-470\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11349260/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jornal Brasileiro de Reproducao Assistida\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5935/1518-0557.20240041\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jornal Brasileiro de Reproducao Assistida","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5935/1518-0557.20240041","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的：甲氨蝶呤（MTX）被广泛用于治疗各种癌症。然而，MTX 会诱发雄性生殖毒性。本研究探讨了臭氧疗法（OT）对减轻 MTX 在小鼠睾丸中毒性作用的影响：24只小鼠分为四组：对照组、OT组（4毫克/千克臭氧）、MTX组（20毫克/千克）和MTX + OT组。评估睾酮水平、组织学变化和氧化应激生物标志物，以评价 OT 的保护作用：结果表明：MTX破坏了生殖上皮，降低了血清睾酮水平，并增强了睾丸组织的氧化应激。然而，用 OT 治疗可减轻这些不良影响。OT 能有效恢复抗氧化酶的水平，如过氧化氢酶（CAT）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）。OT降低了脂质过氧化反应，表现为丙二醛（MDA）水平的下降。OT 可保持正常的精子发生，改善形态计量参数，并减少 MTX 引起的组织学变化。此外，OT 还能有效恢复睾酮水平：结论：OT可通过抑制氧化应激防止MTX诱发的睾丸损伤。
The protective effect of Ozone on the mice testicular damage induced by methotrexate.
Objective: Methotrexate (MTX) is widely administered for the treatment of various cancers. However, MTX induces male reproductive toxicity. In the current study, the effect of ozone therapy (OT) on reducing the toxic effects of MTX in the mouse testicles has been investigated.
Methods: Twenty-four mice were divided into four groups: control, OT (4 mg/kg ozone), MTX (20 mg/kg), and MTX + OT. Testosterone levels, histological changes, and oxidative stress biomarkers were assessed to evaluate the protective effects of OT.
Results: The results demonstrated that MTX disrupted germinal epithelium, reduced serum testosterone levels, and enhanced oxidative stress in testicular tissue. However, treatment with OT attenuated these adverse effects. OT effectively restored the levels of antioxidant enzymes, such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD). OT reduced lipid peroxidation, as indicated by decreased malondialdehyde (MDA) levels. OT preserved normal spermatogenesis, improved morphometric parameters, and reduced histological changes by MTX. Moreover, OT effectively restored testosterone levels.
Conclusions: OT protects against MTX-induced testicular damage by suppressing oxidative stress.
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