Raymond Zhang, Julien Vermot, Riccardo Gherardi, Hajime Fukui, Renee Chow
{"title":"通过心动周期相位匹配分析斑马鱼心脏中的钙信号","authors":"Raymond Zhang, Julien Vermot, Riccardo Gherardi, Hajime Fukui, Renee Chow","doi":"10.21769/BioProtoc.4989","DOIUrl":null,"url":null,"abstract":"Calcium signalling in the endocardium is critical for heart valve development. Calcium ion pulses in the endocardium are generated in response to mechanical forces due to blood flow and can be visualised in the beating zebrafish heart using a genetically encoded calcium indicator such as GCaMP7a. Analysing these pulses is challenging because of the rapid movement of the heart during heartbeat. This protocol outlines an imaging analysis method used to phase-match the cardiac cycle in single z-slice movies of the beating heart, allowing easy measurement of the calcium signal. Key features • Software to synchronise and analyse frames from movies of the beating heart corresponding to a user-defined phase of the cardiac cycle. • Software to measure the fluorescence intensity of the beating heart corresponding to a user-defined region of interest.","PeriodicalId":93907,"journal":{"name":"Bio-protocol","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Calcium Signal Analysis in the Zebrafish Heart via Phase Matching of the Cardiac Cycle\",\"authors\":\"Raymond Zhang, Julien Vermot, Riccardo Gherardi, Hajime Fukui, Renee Chow\",\"doi\":\"10.21769/BioProtoc.4989\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Calcium signalling in the endocardium is critical for heart valve development. Calcium ion pulses in the endocardium are generated in response to mechanical forces due to blood flow and can be visualised in the beating zebrafish heart using a genetically encoded calcium indicator such as GCaMP7a. Analysing these pulses is challenging because of the rapid movement of the heart during heartbeat. This protocol outlines an imaging analysis method used to phase-match the cardiac cycle in single z-slice movies of the beating heart, allowing easy measurement of the calcium signal. Key features • Software to synchronise and analyse frames from movies of the beating heart corresponding to a user-defined phase of the cardiac cycle. • Software to measure the fluorescence intensity of the beating heart corresponding to a user-defined region of interest.\",\"PeriodicalId\":93907,\"journal\":{\"name\":\"Bio-protocol\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bio-protocol\",\"FirstCategoryId\":\"0\",\"ListUrlMain\":\"https://doi.org/10.21769/BioProtoc.4989\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bio-protocol","FirstCategoryId":"0","ListUrlMain":"https://doi.org/10.21769/BioProtoc.4989","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
心内膜中的钙信号对心脏瓣膜的发育至关重要。心内膜中的钙离子脉冲是对血流产生的机械力的反应,可通过基因编码的钙指示剂(如 GCaMP7a)在跳动的斑马鱼心脏中观察到。由于心脏在搏动过程中快速运动,因此分析这些脉冲具有挑战性。本方案概述了一种成像分析方法,用于在心脏跳动的单个 Z 切片影片中对心脏周期进行相位匹配,从而轻松测量钙信号。主要功能 - 用软件同步和分析与用户定义的心动周期相位相对应的心脏跳动影片帧。- 软件可测量与用户定义的感兴趣区域相对应的心脏跳动荧光强度。
Calcium Signal Analysis in the Zebrafish Heart via Phase Matching of the Cardiac Cycle
Calcium signalling in the endocardium is critical for heart valve development. Calcium ion pulses in the endocardium are generated in response to mechanical forces due to blood flow and can be visualised in the beating zebrafish heart using a genetically encoded calcium indicator such as GCaMP7a. Analysing these pulses is challenging because of the rapid movement of the heart during heartbeat. This protocol outlines an imaging analysis method used to phase-match the cardiac cycle in single z-slice movies of the beating heart, allowing easy measurement of the calcium signal. Key features • Software to synchronise and analyse frames from movies of the beating heart corresponding to a user-defined phase of the cardiac cycle. • Software to measure the fluorescence intensity of the beating heart corresponding to a user-defined region of interest.