Jiajun Chen , Dawei Ni , Wei Xu , Wenli Zhang , Wanmeng Mu
{"title":"用于稀有糖类生物合成的酮糖 3-epimerase 的发现、蛋白质工程和异源生产的最新进展","authors":"Jiajun Chen , Dawei Ni , Wei Xu , Wenli Zhang , Wanmeng Mu","doi":"10.1016/j.tifs.2024.104552","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Ketose 3-epimerase (KEase), with its unique C3 epimerization activity and high promiscuity, serves as a crucial biocatalyst for synthesizing various rare sugars. Due to the crucial roles of rare sugars as functional sweeteners or intermediates in the food and pharmaceutical industries, KEase has garnered widespread research attention over the past few decades.</p></div><div><h3>Scope and approach</h3><p>This review begins with a comprehensive overview of methodologies employed in the discovery of novel KEases, continues with a detailed discussion of enzymatic properties and practical applications of KEases, and then focuses on the approaches of protein engineering for optimizing the performance of KEase and strategies of heterologous expression for achieving high-efficiency production of KEase. This review aims to help readers understand the current research progress related to KEase while providing an informed perspective on its future developments.</p></div><div><h3>Key findings and conclusions</h3><p>KEases offer considerable potential in facilitating the accessibility of rare sugars. To fulfill specific application requirements, various enzyme mining methods have been established to obtain KEases with different enzymatic properties. Furthermore, effective strategies have been developed to enhance KEase in terms of both quality and quantity. To further broaden the industrial applicability of KEase for rare sugar biosynthesis, future endeavors should be focused on systematically optimizing each step in the process, from enzyme sourcing to enzyme production, by integrating multiple strategies.</p></div>","PeriodicalId":441,"journal":{"name":"Trends in Food Science & Technology","volume":null,"pages":null},"PeriodicalIF":15.1000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Recent advances in discovery, protein engineering, and heterologous production of ketose 3-epimerase for rare sugar biosynthesis\",\"authors\":\"Jiajun Chen , Dawei Ni , Wei Xu , Wenli Zhang , Wanmeng Mu\",\"doi\":\"10.1016/j.tifs.2024.104552\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Ketose 3-epimerase (KEase), with its unique C3 epimerization activity and high promiscuity, serves as a crucial biocatalyst for synthesizing various rare sugars. Due to the crucial roles of rare sugars as functional sweeteners or intermediates in the food and pharmaceutical industries, KEase has garnered widespread research attention over the past few decades.</p></div><div><h3>Scope and approach</h3><p>This review begins with a comprehensive overview of methodologies employed in the discovery of novel KEases, continues with a detailed discussion of enzymatic properties and practical applications of KEases, and then focuses on the approaches of protein engineering for optimizing the performance of KEase and strategies of heterologous expression for achieving high-efficiency production of KEase. This review aims to help readers understand the current research progress related to KEase while providing an informed perspective on its future developments.</p></div><div><h3>Key findings and conclusions</h3><p>KEases offer considerable potential in facilitating the accessibility of rare sugars. To fulfill specific application requirements, various enzyme mining methods have been established to obtain KEases with different enzymatic properties. Furthermore, effective strategies have been developed to enhance KEase in terms of both quality and quantity. To further broaden the industrial applicability of KEase for rare sugar biosynthesis, future endeavors should be focused on systematically optimizing each step in the process, from enzyme sourcing to enzyme production, by integrating multiple strategies.</p></div>\",\"PeriodicalId\":441,\"journal\":{\"name\":\"Trends in Food Science & Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":15.1000,\"publicationDate\":\"2024-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Trends in Food Science & Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0924224424002280\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Food Science & Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0924224424002280","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Recent advances in discovery, protein engineering, and heterologous production of ketose 3-epimerase for rare sugar biosynthesis
Background
Ketose 3-epimerase (KEase), with its unique C3 epimerization activity and high promiscuity, serves as a crucial biocatalyst for synthesizing various rare sugars. Due to the crucial roles of rare sugars as functional sweeteners or intermediates in the food and pharmaceutical industries, KEase has garnered widespread research attention over the past few decades.
Scope and approach
This review begins with a comprehensive overview of methodologies employed in the discovery of novel KEases, continues with a detailed discussion of enzymatic properties and practical applications of KEases, and then focuses on the approaches of protein engineering for optimizing the performance of KEase and strategies of heterologous expression for achieving high-efficiency production of KEase. This review aims to help readers understand the current research progress related to KEase while providing an informed perspective on its future developments.
Key findings and conclusions
KEases offer considerable potential in facilitating the accessibility of rare sugars. To fulfill specific application requirements, various enzyme mining methods have been established to obtain KEases with different enzymatic properties. Furthermore, effective strategies have been developed to enhance KEase in terms of both quality and quantity. To further broaden the industrial applicability of KEase for rare sugar biosynthesis, future endeavors should be focused on systematically optimizing each step in the process, from enzyme sourcing to enzyme production, by integrating multiple strategies.
期刊介绍:
Trends in Food Science & Technology is a prestigious international journal that specializes in peer-reviewed articles covering the latest advancements in technology, food science, and human nutrition. It serves as a bridge between specialized primary journals and general trade magazines, providing readable and scientifically rigorous reviews and commentaries on current research developments and their potential applications in the food industry.
Unlike traditional journals, Trends in Food Science & Technology does not publish original research papers. Instead, it focuses on critical and comprehensive reviews to offer valuable insights for professionals in the field. By bringing together cutting-edge research and industry applications, this journal plays a vital role in disseminating knowledge and facilitating advancements in the food science and technology sector.