乳腺癌组织中 BECN1 mRNA 的表达;与肿瘤分级显著相关。

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Sarah Ahmed Aglan, Ahmed Mostafa Awad, Yasmine Nagy Elwany, Sanaa Shawky, Radwa Mohamed Abdel Salam, Rasha Said Omar, Rasha Abdel Mawla Ghazala, Nada Ahmed Soliman, Marwa Ibrahim Khedr, Lamia Said Kandil, Mohamed Sultan, Yasser Hamed, Noha Said Kandil
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引用次数: 0

摘要

乳腺癌(BC)是一种异质性疾病,其发病、进展和耐药性涉及多种途径。自噬是一种负责自我消化受损细胞器的细胞过程,已被认为是癌症进展和化疗耐药性的重要参与者。自噬蛋白 Beclin 1(BECN1)的单倍体缺陷被认为是导致癌症发病和进展的原因之一。在我们的研究中,我们调查了BECN1在埃及女性BC患者队列中的表达情况,并通过评估其与2年随访后无病生存期(DFS)的相关性以及与肿瘤临床病理特征的相关性,研究了BECN1的预后作用。研究人员纳入了 20 份冰冻的女性 BC 组织样本和 17 份邻近的正常组织样本,并检测了 BECN1 的表达水平。虽然肿瘤组织的表达量为0.73(0-8.95),低于相应正常组织的表达量1.02(0.04-19.59),但差异无统计学意义(P:0.463)。BECN1 的表达与分期、结节转移和肿瘤大小无关,分别为 0.435、0.541 和 0.296。然而,在研究病例中,分级与 BECN1 mRNA 表达之间存在统计学意义上的负相关,P:0.028。BECN1 表达与 DFS 无统计学意义,P = 0.944。然而,我们观察到三阴性(TNBC)病例的 DFS 率明显低于管腔 BC 患者,P:0.022,平均 DFS 为 19.0 个月,而管腔 BC 患者的平均 DFS 为 23.41 个月。我们的研究强调了BECN1在BC发病机制中的潜在作用,显示BECN1的表达与BC的分化程度相关,表明其可能与疾病的侵袭性有关。随访两年的 DFS 显示,TNBC 亚型仍然与较差的预后相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

BECN1 mRNA expression in breast cancer tissue; significant correlation to tumor grade.

BECN1 mRNA expression in breast cancer tissue; significant correlation to tumor grade.

Breast cancer (BC) is a heterogenous disease with multiple pathways implicated in its development, progression, and drug resistance. Autophagy, a cellular process responsible for self-digestion of damaged organelles, had been recognized as eminent player in cancer progression and chemotherapeutic resistance. The haploinsufficiency of Beclin 1 (BECN1), autophagy protein, is believed to contribute to cancer pathogenesis and progression. In our study, we investigated the expression of BECN1 in a BC female Egyptian patient cohort, as well as its prognostic role through evaluating its association with disease free survival (DFS) after 2 years follow up and association of tumor clinicopathological features. Twenty frozen female BC tissue samples and 17 adjacent normal tissue were included and examined for the expression levels of BECN1. Although the tumor tissues showed lower expression 0.73 (0-8.95) than their corresponding normal tissues 1.02 (0.04-19.59), it was not statistically significant, p: 0.463. BECN1 expression was not associated with stage, nodal metastasis or tumor size, p:0.435, 0.541, 0.296, respectively. However, statistically significant negative correlation was found between grade and BECN1 mRNA expression in the studied cases, p:0.028. BECN1 expression had no statistically significant association with DFS, P = 0.944. However, we observed that triple negative (TNBC) cases had significantly lower DFS rate than luminal BC patients, p: 0.022, with mean DFS 19.0 months, while luminal BC patients had mean DFS of 23.41 months. Our study highlights the potential role of BECN1 in BC pathogenesis, showing that BECN1 expression correlates with poorer differentiation of BC, indicating its probable link with disease aggressiveness. DFS two years follow up showed that TNBC subtype remains associated with less favorable prognosis.

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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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