Einar Martínez-de la Parte, Luis Pérez-Vicente, David E. Torres, Anouk van Westerhoven, Harold J. G. Meijer, Michael F. Seidl, Gert H. J. Kema
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引用次数: 0
摘要
香蕉镰刀菌枯萎病(FWB)是一种严重的植物病害,导致全球香蕉生产遭受重大损失。它仍然是古巴香蕉种植的一个主要问题。香蕉枯萎病是由土壤传播的 Fusarium oxysporum 复合菌种引起的。然而,古巴香蕉所感染的镰刀菌种之间的遗传多样性在很大程度上仍未得到探索。在我们的全面调查中,我们检查了古巴所有生产区有症状的香蕉植株,收集了 170 个镰刀菌分离株。通过基因分型测序和全基因组比较,我们研究了这些分离株的遗传多样性,并将其与全球镰刀菌样本进行了比较。值得注意的是,在 14 个省的 Bluggoe 烹饪香蕉和 Pisang Awak 亚群中观察到了典型的 FWB 症状。我们的系统发育分析表明,F. purpurascens、F. phialophorum 和 F. tardichlamydosporum 是古巴 FWB 的罪魁祸首,其中 F. tardichlamydosporum 在种群中占主导地位。此外,我们还发现了五到七个不同的基因群,其中 F. tardichlamydosporum 分离物至少形成了两个亚群。这一发现强调了造成美洲 FWB 的镰刀菌属的高度遗传多样性。我们的研究揭示了古巴以及更广泛的拉丁美洲和加勒比地区的 FWB 病原体的种群遗传结构和多样性。
Genetic diversity of the banana Fusarium wilt pathogen in Cuba and across Latin America and the Caribbean
Fusarium wilt of bananas (FWB) is a severe plant disease that leads to substantial losses in banana production worldwide. It remains a major concern for Cuban banana cultivation. The disease is caused by members of the soil-borne Fusarium oxysporum species complex. However, the genetic diversity among Fusarium species infecting bananas in Cuba has remained largely unexplored. In our comprehensive survey, we examined symptomatic banana plants across all production zones in the country, collecting 170 Fusarium isolates. Leveraging genotyping-by-sequencing and whole-genome comparisons, we investigated the genetic diversity within these isolates and compared it with a global Fusarium panel. Notably, typical FWB symptoms were observed in Bluggoe cooking bananas and Pisang Awak subgroups across 14 provinces. Our phylogenetic analysis revealed that F. purpurascens, F. phialophorum, and F. tardichlamydosporum are responsible for FWB in Cuba, with F. tardichlamydosporum dominating the population. Furthermore, we identified between five and seven distinct genetic clusters, with F. tardichlamydosporum isolates forming at least two subgroups. This finding underscores the high genetic diversity of Fusarium spp. contributing to FWB in the Americas. Our study sheds light on the population genetic structure and diversity of the FWB pathogen in Cuba and the broader Latin American and Caribbean regions.
期刊介绍:
Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following:
the structure, activities and communal behaviour of microbial communities
microbial community genetics and evolutionary processes
microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors
microbes in the tree of life, microbial diversification and evolution
population biology and clonal structure
microbial metabolic and structural diversity
microbial physiology, growth and survival
microbes and surfaces, adhesion and biofouling
responses to environmental signals and stress factors
modelling and theory development
pollution microbiology
extremophiles and life in extreme and unusual little-explored habitats
element cycles and biogeochemical processes, primary and secondary production
microbes in a changing world, microbially-influenced global changes
evolution and diversity of archaeal and bacterial viruses
new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens