翻译抑制剂和失忆剂依美汀也能抑制正在进行的海马神经活动,与其他蛋白质合成阻断剂类似。

IF 2.4 3区 医学 Q3 NEUROSCIENCES
Hippocampus Pub Date : 2024-05-24 DOI:10.1002/hipo.23611
S. Al-Smadi, A. Padros, G. G. Goss, C. T. Dickson
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引用次数: 0

摘要

人们认为记忆的巩固最终取决于新蛋白质的合成,因为翻译抑制剂(如安诺霉素和环己亚胺)会对长期记忆的持久性产生不利影响。然而,当这些药物直接作用于大脑时,也会严重抑制神经活动,其程度与抑制蛋白质合成的程度直接相关。鉴于神经活动本身可能有助于巩固记忆,这一发现是对严格的新蛋白质记忆假说的严重批评。在这里,我们测试了另一种翻译抑制剂依美汀的神经生理学效应。与对侧海马相比,单侧海马内输注依美汀可抑制同侧部位正在进行的局部场和多单位活动,其方式与自显影证实的蛋白质合成抑制程度呈正相关。这种活动抑制也是发生蛋白质合成抑制的限定脑区所特有的。这些实验进一步证明,持续的蛋白质合成对神经功能是必要的和基本的,并表明在使用转化抑制剂的行为实验中观察到的记忆中断可能在很大程度上是由于神经抑制造成的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The translational inhibitor and amnestic agent emetine also suppresses ongoing hippocampal neural activity similarly to other blockers of protein synthesis

The translational inhibitor and amnestic agent emetine also suppresses ongoing hippocampal neural activity similarly to other blockers of protein synthesis

The consolidation of memory is thought to ultimately depend on the synthesis of new proteins, since translational inhibitors such as anisomycin and cycloheximide adversely affect the permanence of long-term memory. However, when applied directly in brain, these agents also profoundly suppress neural activity to an extent that is directly correlated to the degree of protein synthesis inhibition caused. Given that neural activity itself is likely to help mediate consolidation, this finding is a serious criticism of the strict de novo protein hypothesis of memory. Here, we test the neurophysiological effects of another translational inhibitor, emetine. Unilateral intra-hippocampal infusion of emetine suppressed ongoing local field and multiunit activity at ipsilateral sites as compared to the contralateral hippocampus in a fashion that was positively correlated to the degree of protein synthesis inhibition as confirmed by autoradiography. This suppression of activity was also specific to the circumscribed brain region in which protein synthesis inhibition took place. These experiments provide further evidence that ongoing protein synthesis is necessary and fundamental for neural function and suggest that the disruption of memory observed in behavioral experiments using translational inhibitors may be due, in large part, to neural suppression.

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来源期刊
Hippocampus
Hippocampus 医学-神经科学
CiteScore
5.80
自引率
5.70%
发文量
79
审稿时长
3-8 weeks
期刊介绍: Hippocampus provides a forum for the exchange of current information between investigators interested in the neurobiology of the hippocampal formation and related structures. While the relationships of submitted papers to the hippocampal formation will be evaluated liberally, the substance of appropriate papers should deal with the hippocampal formation per se or with the interaction between the hippocampal formation and other brain regions. The scope of Hippocampus is wide: single and multidisciplinary experimental studies from all fields of basic science, theoretical papers, papers dealing with hippocampal preparations as models for understanding the central nervous system, and clinical studies will be considered for publication. The Editor especially encourages the submission of papers that contribute to a functional understanding of the hippocampal formation.
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