Patrícia Ribeiro Orlando, Hugo Giordano Tavares, Ramona Ramalho de Souza Pereira, Gabriela Silva, Jaqueline do Carmo Lima Carvalho, Alan Rodrigues Teixeira Machado, Leonardo Barros Dobbs, Marco Fabrício Dias-Peixoto, Luciano José Pereira, Eric Francelino Andrade
{"title":"从农业生物质中提取的腐殖酸能减轻牙周炎大鼠的牙槽骨损失并调节全身炎症细胞因子。","authors":"Patrícia Ribeiro Orlando, Hugo Giordano Tavares, Ramona Ramalho de Souza Pereira, Gabriela Silva, Jaqueline do Carmo Lima Carvalho, Alan Rodrigues Teixeira Machado, Leonardo Barros Dobbs, Marco Fabrício Dias-Peixoto, Luciano José Pereira, Eric Francelino Andrade","doi":"10.2174/0115680266302476240510115556","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Humic acid (HA) is a bioproduct that can be extracted from different sources and has anti-inflammatory properties that have been little explored in the treatment and prevention of Periodontal Disease (PD). Thus, we aimed to investigate the effects of oral administration of HA on the progression of PD in rats.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were distributed into three experimental groups (Control/ Sham, PD, and PD + HA). HA was administered by gavage (80 mg/kg/day) for 28 days, and PD was induced 14 days after the beginning of treatment. Bone loss, bone topography, and surface elemental composition were analyzed. Circulating IL1-beta, TNF-alpha, and IL-10 levels were evaluated through Enzyme-Linked Immunosorbent Assay (ELISA).</p><p><strong>Results: </strong>The animals treated with HA showed lower bone loss (p < 0.05). Calcium and phosphorus levels on the alveolar bone surface were lower in the PD group (p < 0.05) compared to the control group, whereas the animals treated with HA exhibited attenuation in this loss (p < 0.05). The animals treated with HA showed reduced TNF-alpha, IL1-beta, IL-10, and the TNF-alpha/IL-10 ratio compared to those with PD (p < 0.05).</p><p><strong>Conclusion: </strong>Treatment with HA attenuated the parameters of alveolar bone loss and modulated systemic inflammatory parameters in rats with ligature-induced PD.</p>","PeriodicalId":11076,"journal":{"name":"Current topics in medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Humic Acid Derived from Agricultural Biomass Mitigates Alveolar Bone Loss and Modulates Systemic Inflammatory Cytokines in Rats with Periodontitis.\",\"authors\":\"Patrícia Ribeiro Orlando, Hugo Giordano Tavares, Ramona Ramalho de Souza Pereira, Gabriela Silva, Jaqueline do Carmo Lima Carvalho, Alan Rodrigues Teixeira Machado, Leonardo Barros Dobbs, Marco Fabrício Dias-Peixoto, Luciano José Pereira, Eric Francelino Andrade\",\"doi\":\"10.2174/0115680266302476240510115556\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Humic acid (HA) is a bioproduct that can be extracted from different sources and has anti-inflammatory properties that have been little explored in the treatment and prevention of Periodontal Disease (PD). Thus, we aimed to investigate the effects of oral administration of HA on the progression of PD in rats.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were distributed into three experimental groups (Control/ Sham, PD, and PD + HA). HA was administered by gavage (80 mg/kg/day) for 28 days, and PD was induced 14 days after the beginning of treatment. Bone loss, bone topography, and surface elemental composition were analyzed. Circulating IL1-beta, TNF-alpha, and IL-10 levels were evaluated through Enzyme-Linked Immunosorbent Assay (ELISA).</p><p><strong>Results: </strong>The animals treated with HA showed lower bone loss (p < 0.05). Calcium and phosphorus levels on the alveolar bone surface were lower in the PD group (p < 0.05) compared to the control group, whereas the animals treated with HA exhibited attenuation in this loss (p < 0.05). The animals treated with HA showed reduced TNF-alpha, IL1-beta, IL-10, and the TNF-alpha/IL-10 ratio compared to those with PD (p < 0.05).</p><p><strong>Conclusion: </strong>Treatment with HA attenuated the parameters of alveolar bone loss and modulated systemic inflammatory parameters in rats with ligature-induced PD.</p>\",\"PeriodicalId\":11076,\"journal\":{\"name\":\"Current topics in medicinal chemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current topics in medicinal chemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2174/0115680266302476240510115556\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current topics in medicinal chemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2174/0115680266302476240510115556","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
摘要
背景：腐植酸（HA）是一种可从不同来源提取的生物制品，具有抗炎特性，但在治疗和预防牙周病（PD）方面却鲜有研究。因此，我们旨在研究口服 HA 对大鼠牙周病进展的影响：将 24 只雄性 Wistar 大鼠分为三个实验组（对照组/ Sham 组、PD 组和 PD + HA 组）。连续 28 天灌胃服用 HA（80 毫克/千克/天），并在治疗开始 14 天后诱发 PD。对骨质流失、骨地形和表面元素组成进行分析。通过酶联免疫吸附试验（ELISA）评估了循环中IL1-β、TNF-α和IL-10的水平：结果：接受 HA 治疗的动物骨质流失较少（p < 0.05）。与对照组相比，PD 组牙槽骨表面的钙和磷水平较低（p < 0.05），而接受 HA 治疗的动物的钙和磷水平下降幅度较小（p < 0.05）。与PD组相比，接受HA治疗的动物TNF-α、IL1-beta、IL-10和TNF-α/IL-10比值均有所降低（P < 0.05）：结论：HA治疗可减轻结扎诱导的PD大鼠牙槽骨损失的参数，并调节全身炎症参数。
Humic Acid Derived from Agricultural Biomass Mitigates Alveolar Bone Loss and Modulates Systemic Inflammatory Cytokines in Rats with Periodontitis.
Background: Humic acid (HA) is a bioproduct that can be extracted from different sources and has anti-inflammatory properties that have been little explored in the treatment and prevention of Periodontal Disease (PD). Thus, we aimed to investigate the effects of oral administration of HA on the progression of PD in rats.
Methods: Twenty-four male Wistar rats were distributed into three experimental groups (Control/ Sham, PD, and PD + HA). HA was administered by gavage (80 mg/kg/day) for 28 days, and PD was induced 14 days after the beginning of treatment. Bone loss, bone topography, and surface elemental composition were analyzed. Circulating IL1-beta, TNF-alpha, and IL-10 levels were evaluated through Enzyme-Linked Immunosorbent Assay (ELISA).
Results: The animals treated with HA showed lower bone loss (p < 0.05). Calcium and phosphorus levels on the alveolar bone surface were lower in the PD group (p < 0.05) compared to the control group, whereas the animals treated with HA exhibited attenuation in this loss (p < 0.05). The animals treated with HA showed reduced TNF-alpha, IL1-beta, IL-10, and the TNF-alpha/IL-10 ratio compared to those with PD (p < 0.05).
Conclusion: Treatment with HA attenuated the parameters of alveolar bone loss and modulated systemic inflammatory parameters in rats with ligature-induced PD.
期刊介绍：
Current Topics in Medicinal Chemistry is a forum for the review of areas of keen and topical interest to medicinal chemists and others in the allied disciplines. Each issue is solely devoted to a specific topic, containing six to nine reviews, which provide the reader a comprehensive survey of that area. A Guest Editor who is an expert in the topic under review, will assemble each issue. The scope of Current Topics in Medicinal Chemistry will cover all areas of medicinal chemistry, including current developments in rational drug design, synthetic chemistry, bioorganic chemistry, high-throughput screening, combinatorial chemistry, compound diversity measurements, drug absorption, drug distribution, metabolism, new and emerging drug targets, natural products, pharmacogenomics, and structure-activity relationships. Medicinal chemistry is a rapidly maturing discipline. The study of how structure and function are related is absolutely essential to understanding the molecular basis of life. Current Topics in Medicinal Chemistry aims to contribute to the growth of scientific knowledge and insight, and facilitate the discovery and development of new therapeutic agents to treat debilitating human disorders. The journal is essential for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important advances.
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