Hajrah A Khan, Leena A Neyaz, Hesham A Malak, Wafa A Alshehri, Khaled Elbanna, Sameer R Organji, Fatimah H Asiri, Mohammad S Aldosari, Hussein H Abulreesh
{"title":"沙特阿拉伯西部临床和环境肠炎沙门氏菌血清型的多样性和抗菌药敏感性模式。","authors":"Hajrah A Khan, Leena A Neyaz, Hesham A Malak, Wafa A Alshehri, Khaled Elbanna, Sameer R Organji, Fatimah H Asiri, Mohammad S Aldosari, Hussein H Abulreesh","doi":"10.1007/s12223-024-01172-1","DOIUrl":null,"url":null,"abstract":"<p><p>The diverse environmental distribution of Salmonella makes it a global source of human gastrointestinal infections. This study aimed to detect Salmonella spp. and explore their diversity and antimicrobial susceptibility patterns in clinical and environmental samples. Pre-enrichment, selective enrichment, and selective plating techniques were adopted for the Salmonella detection whereas the API 20E test and Vitek Compact 2 system were used to confirm the identity of isolates. Salmonella serovars were subjected to molecular confirmation by 16S rDNA gene sequencing. Disc diffusion method and Vitek 2 Compact system determined the antibiotic susceptibility of Salmonella serovars. Multiple antibiotic resistance index (MARI) was calculated to explore whether Salmonella serovars originate from areas with heavy antibiotic usage. Results depicted low Salmonella prevalence in clinical and environmental samples (3.5%). The main detected serovars included Salmonella Typhimurium, S. enteritidis, S. Infantis, S. Newlands, S. Heidelberg, S. Indian, S. Reading, and S. paratyphi C. All the detected Salmonella serovars (27) exhibited multidrug resistance to three or more antimicrobial classes. The study concludes that the overall Salmonella serovars prevalence was found to be low in environmental and clinical samples of Western Saudi Arabia (Makkah and Jeddah). However, antimicrobial susceptibility patterns of human and environmental Salmonella serovars revealed that all isolates exhibited multidrug-resistance (MDR) patterns to frequently used antibiotics, which might reflect antibiotic overuse in clinical and veterinary medicine. It would be suitable to apply and enforce rules and regulations from the One Health approach, which aim to prevent antibiotic resistance infections, enhance food safety, and improve human and animal health, given that all Salmonella spp. detected in this investigation were exhibiting MDR patterns.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"1305-1317"},"PeriodicalIF":2.4000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Diversity and antimicrobial susceptibility patterns of clinical and environmental Salmonella enterica serovars in Western Saudi Arabia.\",\"authors\":\"Hajrah A Khan, Leena A Neyaz, Hesham A Malak, Wafa A Alshehri, Khaled Elbanna, Sameer R Organji, Fatimah H Asiri, Mohammad S Aldosari, Hussein H Abulreesh\",\"doi\":\"10.1007/s12223-024-01172-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The diverse environmental distribution of Salmonella makes it a global source of human gastrointestinal infections. 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All the detected Salmonella serovars (27) exhibited multidrug resistance to three or more antimicrobial classes. The study concludes that the overall Salmonella serovars prevalence was found to be low in environmental and clinical samples of Western Saudi Arabia (Makkah and Jeddah). However, antimicrobial susceptibility patterns of human and environmental Salmonella serovars revealed that all isolates exhibited multidrug-resistance (MDR) patterns to frequently used antibiotics, which might reflect antibiotic overuse in clinical and veterinary medicine. 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引用次数: 0
摘要
沙门氏菌在环境中的多样化分布使其成为人类胃肠道感染的全球来源。本研究旨在检测临床和环境样本中的沙门氏菌属,并探索其多样性和抗菌药敏感性模式。沙门氏菌的检测采用了预富集、选择性富集和选择性平板技术,而 API 20E 试验和 Vitek Compact 2 系统则用于确认分离菌株的身份。通过 16S rDNA 基因测序对沙门氏菌血清进行分子确认。圆盘扩散法和 Vitek 2 Compact 系统确定了沙门氏菌血清种的抗生素敏感性。通过计算多重抗生素耐药性指数(MARI)来探究沙门氏菌血清型是否来自抗生素使用量大的地区。结果显示,沙门氏菌在临床和环境样本中的流行率较低(3.5%)。检测到的主要血清型包括鼠伤寒沙门氏菌、肠炎沙门氏菌、因凡蒂斯沙门氏菌、纽兰兹沙门氏菌、海德堡沙门氏菌、印度沙门氏菌、雷丁沙门氏菌和副伤寒 C 沙门氏菌。所有检测到的沙门氏菌血清型(27 个)都表现出对三种或三种以上抗菌药物的多重耐药性。研究得出结论:在沙特阿拉伯西部(麦加和吉达)的环境和临床样本中,沙门氏菌血清型的总体流行率较低。不过,人类和环境沙门氏菌血清型的抗菌药敏感性模式显示,所有分离菌株都表现出对常用抗生素的多重耐药性(MDR)模式,这可能反映了临床和兽医中抗生素的过度使用。鉴于本次调查中检测到的所有沙门氏菌属都表现出 MDR 模式,因此适合应用和执行旨在预防抗生素耐药性感染、加强食品安全和改善人类及动物健康的 "同一健康 "方法中的规则和条例。
Diversity and antimicrobial susceptibility patterns of clinical and environmental Salmonella enterica serovars in Western Saudi Arabia.
The diverse environmental distribution of Salmonella makes it a global source of human gastrointestinal infections. This study aimed to detect Salmonella spp. and explore their diversity and antimicrobial susceptibility patterns in clinical and environmental samples. Pre-enrichment, selective enrichment, and selective plating techniques were adopted for the Salmonella detection whereas the API 20E test and Vitek Compact 2 system were used to confirm the identity of isolates. Salmonella serovars were subjected to molecular confirmation by 16S rDNA gene sequencing. Disc diffusion method and Vitek 2 Compact system determined the antibiotic susceptibility of Salmonella serovars. Multiple antibiotic resistance index (MARI) was calculated to explore whether Salmonella serovars originate from areas with heavy antibiotic usage. Results depicted low Salmonella prevalence in clinical and environmental samples (3.5%). The main detected serovars included Salmonella Typhimurium, S. enteritidis, S. Infantis, S. Newlands, S. Heidelberg, S. Indian, S. Reading, and S. paratyphi C. All the detected Salmonella serovars (27) exhibited multidrug resistance to three or more antimicrobial classes. The study concludes that the overall Salmonella serovars prevalence was found to be low in environmental and clinical samples of Western Saudi Arabia (Makkah and Jeddah). However, antimicrobial susceptibility patterns of human and environmental Salmonella serovars revealed that all isolates exhibited multidrug-resistance (MDR) patterns to frequently used antibiotics, which might reflect antibiotic overuse in clinical and veterinary medicine. It would be suitable to apply and enforce rules and regulations from the One Health approach, which aim to prevent antibiotic resistance infections, enhance food safety, and improve human and animal health, given that all Salmonella spp. detected in this investigation were exhibiting MDR patterns.
期刊介绍:
Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.