楠木(Artocarpus heteropyllus L )抗逆性的分析与 FRAP 试验

S. Sulasmi
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摘要

背景:抗氧化剂是一种能够抵消或减少体内氧化剂负面影响的化合物。抗氧化剂的活性就是胡柚叶抵御自由基的能力。菠萝蜜叶(Artocarpus heteropyllus L.)属于桑科,含有次生代谢物。研究结果表明,菠萝蜜叶可用作天然抗氧化剂,因为它们含有次生代谢物,即黄酮类、皂苷和单宁酸。目的:本研究使用紫外可见分光光度法(Uv-Vis),用氯化铁试剂测定千层塔叶提取物的抗氧化活性水平。方法:本研究采用实验法,用 96% 的乙醇浸渍萃取柚皮叶,然后用旋转蒸发仪蒸发滤液。用紫外可见分光光度法在 400 nm-800 nm 范围内,用氯化铁试剂分析菠萝叶提取物的抗氧化活性。结果:通过对菠萝蜜粉进行植物化学筛选,获得了黄酮类化合物、皂苷和单宁酸。在 236 纳米波长下测定抗坏血酸的抗氧化水平为 87.4712 毫克 QE/克,在 400 纳米波长下测定菠萝叶的抗氧化水平为 1.2736 毫克 AAE/克。结论:结果表明,柚子叶的 IC50 值为 74.6 ppm。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ANALISIS ANTIOKSIDAN EKSTRAK DAUN NANGKA (Artocarpus heteropyllus L ) DENGAN MENGGUNAKAN METODE FRAP
Background: Antioxidants are compounds that are able to counteract or reduce the negative effects of oxidants in the body. The activity of antioxidants is the ability of jackfruit leaves to ward off free radicals. Jackfruit leaf (Artocarpus heteropyllus L.) belongs to the Moraceae family which contains secondary metabolites. The results showed that jackfruit leaves are used as natural antioxidants because they contain secondary metabolites, namely flavonoids, saponins and tannins. Purpose : of this study was to determine the level of antioxidant activity of jackfruit leaf extract using ferric chloride reagent using Ultraviolet-Visible (Uv-Vis) spectrophotometry. Methods : This study used an experimental method, simplicia was extracted by maceration using 96% ethanol and the filtrate obtained was evaporated with the help of a rotary evaporator. Jackfruit leaf extract was analyzed for antioxidant activity with ferric chloride reagent using UV-Vis spectrophotometry in the range of 400 nm-800 nm. Results : of phytochemical screening of jackfruit simplicia powder obtained flavonoid compounds, saponins and tannins. The of the determination of antioxidant levels of ascorbic acid at a wavelength of 236 nm with levels of 87.4712 mgQE/g and levels of jackfruit leaves at a wavelength of 400 nm with levels of 1.2736 mg AAE/g. Conclusion : The results showed that the IC50 value of jackfruit leaves had a value of 74.6 ppm.
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