FORMULATION OF NANOPARTICLE CONTAINING KENIKIR LEAF ETHANOL EXTRACTS (COSMOS CAUDATUS KUNTH.) AND ANTIDIABETIC ACTIVITY IN RATS

Objective: This study aimed to formulate the ethanol extract of kenikir leaf into nanoparticles. The ethanol extract of kenikir leaves loading into nanoparticles can enhanced the stability and effectiveness of antidiabetic activity. Methods: The nanoparticles were prepared using the ionic gelation method with chitosan and variation in sodium tripolyphosphate. The nanoparticle formula was characterized by efficiency encapsulation using spectrophotometry methods and particle size, zeta potential, and polydispersity index using dynamic light scattering (DLS). An antidiabetic activity test was initiated by inducing a high-fat and fructose diet. The parameters tested were decreasing blood glucose levels in rats. Results: The result of the characterization of the nanoparticle was the percent of efficiency encapsulation, particle size, PDI, zeta potential, and pH were carried out to get the best formula. The best formula obtained was the percent of efficiency encapsulation of 96.20±0.0278%, the particle size of 144.6±7.800 nm, zeta potential of+15.32±0.9550 mEv, PDI of 0.48±0.070, and pH of 4.255±0.0035. The decrease in blood glucose levels in the nanoparticles of kenikir leaves extract was not significantly (p>0.05) different from the positive group (metformin) compared to the kenikir leaves extract, which decreased not really significantly. Conclusion: Nanoparticle containing kenikir leaf ethanol extract successfully prepared into nanoparticles and the potential to increase antidiabetic activity.